Evaluation of different selective media for enumeration of probiotic micro-organisms in combination with yogurt starter cultures in fermented milk


Autoria(s): Saccaro, Daniela Marques; Hirota, Claudia Y.; Tamime, Adnan Y.; de Oliveira, Marice Nogueira
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

06/11/2013

06/11/2013

2012

Resumo

The aim of this study was to assess selective plating methodologies for the enumeration and identification of Streptococcus thermophilus, Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus, Lactobacillus rhamnosus and Bifidobacterium animalis ssp. lactis in fermented milks. Seven agar media (MRS with added sorbitol, clindamycin or vancomycin, acidified MRS, RCA with added aniline blue and dicloxacilin, M17 and ST) were evaluated. The results showed that RCA dicloxacilin agar was suitable for the selective enumeration of B. animalis ssp. lactis in fermented milk. Either MRS (acidified) or M17 agar could be used for enumeration of L. delbrueckii ssp. bulgaricus and S. thermophilus, respectively. MRS media containing antibiotics were effective for the enumeration of the probiotic organisms (L. rhamnosus and L. acidophilus) inoculated in fermented milks.

FAPESP

FAPESP

CNPq

CNPq

Identificador

AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, VICTORIA ISLAND, v. 6, n. 10, supl. 1, Part 1, pp. 2239-2245, 42430, 2012

1996-0808

http://www.producao.usp.br/handle/BDPI/42347

10.5897/AJMR11.117

http://dx.doi.org/10.5897/AJMR11.117

Idioma(s)

eng

Publicador

ACADEMIC JOURNALS

VICTORIA ISLAND

Relação

AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH

Direitos

openAccess

Copyright ACADEMIC JOURNALS

Palavras-Chave #SELECTIVE MEDIA #PROBIOTIC #STARTER CULTURES #FERMENTED MILK #DELBRUECKII SSP BULGARICUS #ANIMALIS SUBSP LACTIS #LACTOBACILLUS-ACIDOPHILUS #BIFIDOBACTERIUM-ANIMALIS #DAIRY-PRODUCTS #STREPTOCOCCUS-THERMOPHILUS #BACTERIA #SURVIVAL #STORAGE #CASEI #MICROBIOLOGY
Tipo

article

original article

publishedVersion