DEVELOPMENT OF MICROSATELLITE MARKERS FOR ANADENANTHERA COLUBRINA (LEGUMINOSAE), A NEOTROPICAL TREE SPECIES


Autoria(s): Feres, Juliana Massimino; Monteiro, Mariza; Zucchi, Maria I.; Pinheiro, José Baldin; Mestriner, Moacyr Antonio; Alzate-Marin, Ana Lilia
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

07/11/2013

07/11/2013

2012

Resumo

Premise of the study: We developed and characterized nuclear microsatellite markers for Anadenanthera colubrina, a tropical tree species widely distributed in South America. Methods and Results: Leaf samples of mature A. colubrina trees, popularly called "angico," were collected from an area that is greatly impacted by agricultural practices in the region of Ribeirao Preto in Sao Paulo State in southeastern Brazil. Twenty simple sequence repeat (SSR) markers were developed, 14 of which had polymorphic loci. A total of 96 alleles were detected with an average of 6.86 alleles per polymorphic locus. The expected heterozygosity, calculated at polymorphic loci, ranged from 0.18 to 0.83. Finally, we demonstrated that 18 loci were cross-amplified in A. peregrina. Conclusions: A total of 14 polymorphic markers suggest a high potential for genetic diversity, gene flow, and mating system analyses in A. colubrina.

FAPESP [2009/14200-6]

CNPq [PDS 150277/2009-1, PV 300140/2011-8]

Identificador

AMERICAN JOURNAL OF BOTANY, ST LOUIS, v. 99, n. 4, p. E154-E156, APR, 2012

0002-9122

http://www.producao.usp.br/handle/BDPI/43174

10.3732/ajb.1100446

http://dx.doi.org/10.3732/ajb.1100446

Idioma(s)

eng

Publicador

BOTANICAL SOC AMER INC

ST LOUIS

Relação

AMERICAN JOURNAL OF BOTANY

Direitos

restrictedAccess

Copyright BOTANICAL SOC AMER INC

Palavras-Chave #ANADENANTHERA COLUBRINA #ANGICO #FOREST CONSERVATION #GENETIC DIVERSITY #LEGUMINOSAE #SIMPLE SEQUENCE REPEAT MARKERS #PLANT SCIENCES
Tipo

article

original article

publishedVersion