Eye enucleation activates the transcription nuclear factor kappa-B in the rat superior colliculus

Ocular enucleation produces significant morphological and physiological changes in central visual areas. However, our knowledge of the molecular events resulting from eye enucleation in visual brain areas remains elusive. We characterized here the transcription nuclear factor kappa-B (NF-kappa B) activation induced by ocular enucleation in the rat superior colliculus (SC). We also tested the effectiveness of the synthetic glucocorticoid dexamethasone in inhibiting its activation. Electrophoretic mobility shift assays to detect NF-kappa B indicated that this transcription factor is activated in the SC from 1 h to day 15 postlesion. The expression of p65 and p50 proteins in the nuclear extracts was also increased. Dexamethasone treatment was able to significantly inhibit NF-kappa B activation. These findings suggest that this transcriptional factor is importantly involved in the visual system short-term processes that ensue after retinal lesions in the adult brain. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)

CAPES (Coordenacao de Aperfeicoamento de Pessoal de Ensino Superior)

CAPES (Coordenacao de Aperfeicoamento de Pessoal de Ensino Superior)

University of Sao Paulo-NAPNA

University of Sao PauloNAPNA

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)