SK-HEP cells and lentiviral vector for production of human recombinant factor VIII
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
|---|---|
| Data(s) |
24/09/2013
24/09/2013
2012
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| Resumo |
Hemophilia A is caused by a deficiency in coagulation factor VIII. Recombinant factor VIII can be used as an alternative although it is unavailable for most patients. Here, we describe the production of a human recombinant B-domain-deleted FVIII (rBDDFVIII) by the human cell line SK-HEP-1, modified by a lentiviral vector rBDDFVIII was produced by recombinant SK-HEP cells (rSK-HEP) at 1.5-2.1 IU/10(6) in 24 h. The recombinant factor had increased in vitro stability when compared to commercial pdFVIII. The functionality of rBDDFVIII was shown by its biological activity and by tail-clip challenge in hemophilia A mice. The rSK-HEP cells grew in a scalable system and produced active rBDDFVIII, indicating that this platform production can be optimized to meet the commercial production scale needs. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) |
| Identificador |
BIOTECHNOLOGY LETTERS, DORDRECHT, v. 34, n. 8, pp. 1435-1443, AUG, 2012 0141-5492 http://www.producao.usp.br/handle/BDPI/33648 10.1007/s10529-012-0925-4 |
| Idioma(s) |
eng |
| Publicador |
SPRINGER DORDRECHT |
| Relação |
BIOTECHNOLOGY LETTERS |
| Direitos |
closedAccess Copyright SPRINGER |
| Palavras-Chave | #HUMAN CELL LINE #HUMAN FACTOR VIII #LENTIVIRAL VECTOR #MICROCARRIER CULTURE #SK-HEP CELLS #RECOMBINANT FACTOR VIII #COAGULATION-FACTOR-VIII #HEMOPHILIA-A #EXPRESSION #GENE #LINE #BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Tipo |
article original article publishedVersion |
