Proteomic analysis of papaya fruit ripening using 2DE-DIGE


Autoria(s): Nogueira, Silvia Beserra; Labate, Carlos Alberto; Gozzo, Fabio Cesar; Pilau, Eduardo Jorge; Lajolo, Franco Maria; Nascimento, João Roberto Oliveira do
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

07/11/2013

07/11/2013

2012

Resumo

Papayas have a very short green life as a result of their rapid pulp softening as well as their susceptibility to physical injury and mold growth. The ripening-related changes take place very quickly, and there is a continued interest in the reduction of postharvest losses. Proteins have a central role in biological processes, and differential proteomics enables the discrimination of proteins affected during papaya ripening. A comparative analysis of the proteomes of climacteric and pre-climacteric papayas was performed using 2DE-DIGE. Third seven proteins corresponding to spots with significant differences in abundance during ripening were submitted to MS analysis, and 27 proteins were identified and classified into six main categories related to the metabolic changes occurring during ripening. Proteins from the cell wall (alpha-galactosidase and invertase), ethylene biosynthesis (methionine synthase), climacteric respiratory burst, stress response, synthesis of carotenoid precursors (hydroxymethylbutenyl 4-diphosphate synthase, GcpE), and chromoplast differentiation (fibrillin) were identified. There was some correspondence between the identified proteins and the data from previous transcript profiling of papaya fruit, but new, accumulated proteins were identified, which reinforces the importance of differential proteomics as a tool to investigate ripening and provides potentially useful information for maintaining fruit quality and minimizing postharvest losses. (C) 2011 Elsevier B.V. All rights reserved.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2008/52447-0]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

CNPq

CNPq

Identificador

JOURNAL OF PROTEOMICS, AMSTERDAM, v. 75, n. 4, pp. 1428-1439, FEB 2, 2012

1874-3919

http://www.producao.usp.br/handle/BDPI/43069

10.1016/j.jprot.2011.11.015

http://dx.doi.org/10.1016/j.jprot.2011.11.015

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

AMSTERDAM

Relação

JOURNAL OF PROTEOMICS

Direitos

closedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #PAPAYA RIPENING #PAPAYA PROTEOME #2DE-DIGE #GCPE #FIBRILLIN #MEP PATHWAY #TOMATO FRUIT #PROTEIN EXPRESSION #COLD-STORAGE #GRAPE BERRY #ETHYLENE #STRESS #ACCUMULATION #MATURATION #INVERTASE #SYNTHASE #BIOCHEMICAL RESEARCH METHODS
Tipo

article

original article

publishedVersion