Partial Replacement of omega-6 Fatty Acids With Medium-Chain Triglycerides, but Not Olive Oil, Improves Colon Cytokine Response and Damage in Experimental Colitis


Autoria(s): Bertevello, Pedro L.; De Nardi, Leticia; Torrinhas, Raquel S.; Logullo, Angela F.; Waitzberg, Dan L.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

11/10/2013

11/10/2013

01/07/2012

Resumo

Background: Soybean oil is rich in omega-6 fatty acids, which are associated with higher incidence and more severe cases of inflammatory bowel diseases. The authors evaluated whether partial replacement of soybean oil by medium-chain triglycerides (MCTs) or olive oil influenced the incidence and severity of experimental ulcerative colitis by using different parenteral lipid emulsions (LEs). Methods: Wistar rats (n = 40) were randomized to receive parenteral infusion of the following LE: 100% soybean oil (SO), 50% MCT mixed with 50% soybean oil (MCT/SO), 80% olive oil mixed with 20% soybean oil (OO/SO), or saline (CC). After 72 hours of infusion, acetic acid experimental colitis was induced. After 24 hours, colon histology and cytokine expression were analyzed. Results: SO was not significantly associated with overall tissue damage. MCT/SO was not associated with necrosis (P < .005), whereas OO/SO had higher frequencies of ulcer and necrosis (P < .005). SO was associated with increased expression of interferon-gamma (P = .005) and OO/SO with increased interleukin (IL)-6 and decreased tumor necrosis factor-alpha expression (P < .05). MCT/SO appeared to decrease IL-1 (P < .05) and increase IL-4 (P < .001) expression. Conclusions: Parenteral SO with high concentration of omega-6 fatty acids was not associated with greater tissue damage in experimental colitis. SO partial replacement with MCT/SO decreased the frequency of histological necrosis and favorably modulated cytokine expression in the colon; however, replacement with OO/SO had unfavorable effects. (JPEN J Parenter Enteral Nutr. 2012; 36: 442-448)

National Council of Scientific and Technological Development (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico

National Council of Scientific and Technological Development (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [CNPq] [140925/00-7]

Sao Paulo State Research Support Foundation (Fundacao de Apoio a Pesquisa do Estado de Sao Paulo [Fapesp], Brazil [97/07971-70]

Sao Paulo State Research Support Foundation (Fundacao de Apoio a Pesquisa do Estado de Sao Paulo , Brazil

Identificador

JOURNAL OF PARENTERAL AND ENTERAL NUTRITION, THOUSAND OAKS, v. 36, n. 4, pp. 442-448, JUL, 2012

0148-6071

http://www.producao.usp.br/handle/BDPI/34125

10.1177/0148607111421788

http://dx.doi.org/10.1177/0148607111421788

Idioma(s)

eng

Publicador

SAGE PUBLICATIONS INC

THOUSAND OAKS

Relação

JOURNAL OF PARENTERAL AND ENTERAL NUTRITION

Direitos

openAccess

Copyright SAGE PUBLICATIONS INC

Palavras-Chave #MEDIUM-CHAIN TRIGLYCERIDES #OLIVE OIL #SOYBEAN OIL #COLITIS #LIPID EMULSION #PARENTERAL NUTRITION #INFLAMMATORY-BOWEL-DISEASE #PARENTERAL LIPID EMULSIONS #OXYGEN RADICAL PRODUCTION #FISH-OIL #ULCERATIVE-COLITIS #HUMAN NEUTROPHILS #DIETARY LIPIDS #CELL FUNCTIONS #ENRICHED DIET #RATS #NUTRITION & DIETETICS
Tipo

article

original article

publishedVersion