Hypertonic saline solution reduces the inflammatory response in endotoxemic rats


Autoria(s): Theobaldo, Mariana Cardillo; Barbeiro, Hermes Vieira; Barbeiro, Denise Frediani; Petroni, Ricardo Costa; Soriano, Francisco Garcia
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

05/11/2013

05/11/2013

2012

Resumo

OBJECTIVE: Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS: We measured cytokines (serum and gut), nitrite, and lipid peroxidation (TBARS) as indicators of oxidative stress in the gut. Rats were divided into four groups: control group (C) that did not receive lipopolysaccharide; lipopolysaccharide injection without treatment (LPS); lipopolysaccharide injection with saline treatment (LPS + S); and lipopolysaccharide injection with hypertonic saline treatment (LPS + H). Serum and intestine were collected. Measurements were taken at 1.5, 8, and 24 h after lipopolysaccharide administration. RESULTS: Of the four groups, the LPS + H group had the highest survival rate. Hypertonic saline solution treatment led to lower levels of IL-6, IL-10, nitric oxide, and thiobarbituric acid reactive substances compared to 0.9% normal saline. In addition, hypertonic saline treatment resulted in a lower mortality compared to 0.9% normal saline treatment in endotoxemic rats. Volume replacement reduced levels of inflammatory mediators in the plasma and gut. CONCLUSION: Hypertonic saline treatment reduced mortality and lowered levels of inflammatory mediators in endotoxemic rats. Hypertonic saline also has the advantage of requiring less volume replacement.

FAPESP-09/03338-7

FAPESP-06/00443-6

CNPQ-470744/2004-9

Identificador

CLINICS, SAO PAULO, v. 67, n. 12, pp. 1463-1468, DEC, 2012

1807-5932

http://www.producao.usp.br/handle/BDPI/41216

10.6061/clinics/2012(12)18

http://dx.doi.org/10.6061/clinics/2012(12)18

Idioma(s)

eng

Publicador

HOSPITAL CLINICAS, UNIV SAO PAULO

SAO PAULO

Relação

CLINICS

Direitos

openAccess

Copyright HOSPITAL CLINICAS, UNIV SAO PAULO

Palavras-Chave #LPS #HYPERTONIC SOLUTION #INTERLEUKIN #OXIDATIVE STRESS #INTESTINE #SEPTIC SHOCK #POLYMICROBIAL SEPSIS #ACUTE-PANCREATITIS #INTERLEUKIN-10 #NEUTROPHILS #EXPRESSION #IL-10 #P38 #MEDICINE, GENERAL & INTERNAL
Tipo

article

original article

publishedVersion