Hypertonic saline solution reduces the inflammatory response in endotoxemic rats
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
05/11/2013
05/11/2013
2012
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Resumo |
OBJECTIVE: Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS: We measured cytokines (serum and gut), nitrite, and lipid peroxidation (TBARS) as indicators of oxidative stress in the gut. Rats were divided into four groups: control group (C) that did not receive lipopolysaccharide; lipopolysaccharide injection without treatment (LPS); lipopolysaccharide injection with saline treatment (LPS + S); and lipopolysaccharide injection with hypertonic saline treatment (LPS + H). Serum and intestine were collected. Measurements were taken at 1.5, 8, and 24 h after lipopolysaccharide administration. RESULTS: Of the four groups, the LPS + H group had the highest survival rate. Hypertonic saline solution treatment led to lower levels of IL-6, IL-10, nitric oxide, and thiobarbituric acid reactive substances compared to 0.9% normal saline. In addition, hypertonic saline treatment resulted in a lower mortality compared to 0.9% normal saline treatment in endotoxemic rats. Volume replacement reduced levels of inflammatory mediators in the plasma and gut. CONCLUSION: Hypertonic saline treatment reduced mortality and lowered levels of inflammatory mediators in endotoxemic rats. Hypertonic saline also has the advantage of requiring less volume replacement. FAPESP-09/03338-7 FAPESP-06/00443-6 CNPQ-470744/2004-9 |
Identificador |
CLINICS, SAO PAULO, v. 67, n. 12, pp. 1463-1468, DEC, 2012 1807-5932 http://www.producao.usp.br/handle/BDPI/41216 10.6061/clinics/2012(12)18 |
Idioma(s) |
eng |
Publicador |
HOSPITAL CLINICAS, UNIV SAO PAULO SAO PAULO |
Relação |
CLINICS |
Direitos |
openAccess Copyright HOSPITAL CLINICAS, UNIV SAO PAULO |
Palavras-Chave | #LPS #HYPERTONIC SOLUTION #INTERLEUKIN #OXIDATIVE STRESS #INTESTINE #SEPTIC SHOCK #POLYMICROBIAL SEPSIS #ACUTE-PANCREATITIS #INTERLEUKIN-10 #NEUTROPHILS #EXPRESSION #IL-10 #P38 #MEDICINE, GENERAL & INTERNAL |
Tipo |
article original article publishedVersion |