Enzymatic Solid-Phase Reactor Based on Silica Organofunctionalized with p-Phenylenediamine for Electrochemical Detection of Phenolic Compounds
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
05/11/2013
05/11/2013
2012
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Resumo |
A new biomaterial, based on silica organofunctionalized with p-phenylenediamine (p-PDA) and the enzyme peroxidase, was used in the development of an enzymatic solid-phase reactor. The analytical techniques used in the characterization showed that the organic ligand was incorporated into the silica matrix. Thus, the silica modified with p-PDA allowed the incorporation of peroxidase by the electrostatic interaction between the carboxylic groups present in the enzyme molecules and the amino groups attached to the silica. The enzymatic solid-phase reactor was used for chemical oxidation of phenols in 1, 4-benzoquinone that was then detected by chronoamperometry. The system allowed the analysis of hydroquinone with a detection limit of 83.6 nmol L-1. Thus, the new material has potential in the determination of phenolic compounds river water samples. FAPESP FAPESP [2010/11049-2, 2010/11567-3] |
Identificador |
SENSOR LETTERS, VALENCIA, v. 10, n. 41367, supl. 1, Part 3, pp. 1031-1038, MAR-APR, 2012 1546-198X http://www.producao.usp.br/handle/BDPI/41656 10.1166/sl.2012.2345 |
Idioma(s) |
eng |
Publicador |
AMER SCIENTIFIC PUBLISHERS VALENCIA |
Relação |
SENSOR LETTERS |
Direitos |
closedAccess Copyright AMER SCIENTIFIC PUBLISHERS |
Palavras-Chave | #P-PHENYLENEDIAMINE #ENZYMATIC SOLID-PHASE REACTOR #PHENOLIC COMPOUNDS #SPHERICAL MESOPOROUS SILICA #COMPOSITE ELECTRODE #CARBON NANOTUBES #MOLECULAR-SIEVES #NATURAL-WATER #MERCURY IONS #THIN-FILMS #HYDROQUINONE #BIOSENSOR #SURFACES #CHEMISTRY, ANALYTICAL #ELECTROCHEMISTRY #INSTRUMENTS & INSTRUMENTATION #PHYSICS, APPLIED |
Tipo |
article original article publishedVersion |