Opioid mu-receptors in the rostral medullary raphe modulate hypoxia-induced hyperpnea in unanesthetized rats


Autoria(s): Dias, M. B.; Nucci, T. B.; Branco, L. G. S.; Gargaglioni, L. H.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

29/10/2013

29/10/2013

2012

Resumo

Aim: It has been suggested that the medullary raphe (MR) plays a key role in the physiological responses to hypoxia. As opioid mu-receptors have been found in the MR, we studied the putative role of opioid mu-receptors in the rostral MR (rMR) region on ventilation in normal and 7% hypoxic conditions. Methods: We measured pulmonary ventilation ((V) over dotE) and the body temperatures (Tb) of male Wistar rats before and after the selective opioid l-receptor antagonist CTAP ( d-Phe-Cys-Tyr-d-Trp-Arg-Thr-Pen-Thr-NH2, cyclic, 0.1 mu g per 0.1 mu L) was microinjected into the rMR during normoxia or after 60 min of hypoxia. Results: The animals treated with intra-rMR CTAP exhibited an attenuation of the ventilatory response to hypoxia ( 430 +/- 86 mL kg) 1 min) 1) compared with the control group ( 790 +/- 82 mL kg) 1 min) 1) ( P < 0.05). No differences in the Tb were observed between groups during hypoxia. Conclusion: These data suggest that opioids acting on l-receptors in the rMR exert an excitatory modulation of hyperventilation induced by hypoxia.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

INCTFisiologia Comparada

INCT-Fisiologia Comparada

Programa de Apoio para grupos de excelencia (PRONEX)

Programa de Apoio para grupos de excelencia (PRONEX)

Identificador

ACTA PHYSIOLOGICA, MALDEN, v. 204, n. 3, supl. 1, Part 3, pp. 435-442, MAR, 2012

1748-1708

http://www.producao.usp.br/handle/BDPI/36306

10.1111/j.1748-1716.2011.02345.x

http://dx.doi.org/10.1111/j.1748-1716.2011.02345.x

Idioma(s)

eng

Publicador

WILEY-BLACKWELL

MALDEN

Relação

ACTA PHYSIOLOGICA

Direitos

closedAccess

Copyright WILEY-BLACKWELL

Palavras-Chave #CONTROL OF BREATHING #OPIATES #PULMONARY VENTILATION #RAPHE NUCLEI #INDUCED RESPIRATORY DEPRESSION #ANTEROVENTRAL PREOPTIC REGION #PRE-BOTZINGER COMPLEX #VENTILATORY RESPONSE #SEROTONINERGIC RECEPTORS #METABOLIC RESPONSES #ANESTHETIZED RATS #MORPHINE #HYPERCAPNIA #NUCLEUS #PHYSIOLOGY
Tipo

article

original article

publishedVersion