CHARACTERIZATION OF LYOPHILIZED LIPOSOMES PRODUCED WITH NON-PURIFIED SOY LECITHIN: A CASE STUDY OF CASEIN HYDROLYSATE MICROENCAPSULATION


Autoria(s): Yokota, D.; Moraes, M.; Pinho, S. C.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

05/11/2013

05/11/2013

2012

Resumo

A commercial casein hydrolysate was microencapsulated in liposomes produced with non-purified soy lecithin, cryoprotected with two different disaccharides and lyophilized. The encapsulation efficiency of casein hydrolysate ranged from 30 to 40%. The powders were analyzed by differential scanning calorimetry (DSC), scanning electron micrography (SEM), infrared spectroscopy (FTIR) and wide angle X-ray diffraction (WAXD). DSC data revealed the presence of an exothermal transition in empty lyophilized liposomes, which was ascribed to the presence of a quasicrystalline lamellar phase (intermediary characteristics between the L-beta and L-c phases). The addition of peptides to the liposomal system caused the disappearance of this exothermic phenomenon, as they were located in the polar headgroup portion of the bilayer, causing disorder and preventing the formation of the quasicrystalline phase. Infrared data indicated the presence of the peptides in the lyophilized formulations and showed that the cryoprotectants interacted effectively with the polar heads of phospholipids in the bilayer.

CNPq - Conselho Nacional de Pesquisa e Desenvolvimento (Brazil)

CNPq Conselho Nacional de Pesquisa e Desenvolvimento (Brazil)

Identificador

BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING, SAO PAULO, v. 29, n. 2, supl., Part 2, pp. 325-335, APR-JUN, 2012

0104-6632

http://www.producao.usp.br/handle/BDPI/41576

10.1590/S0104-66322012000200013

http://dx.doi.org/10.1590/S0104-66322012000200013

Idioma(s)

eng

Publicador

BRAZILIAN SOC CHEMICAL ENG

SAO PAULO

Relação

BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING

Direitos

openAccess

Copyright BRAZILIAN SOC CHEMICAL ENG

Palavras-Chave #CASEIN HYDROLYSATE #LIPOSOMES #LYOPHILIZED LIPOSOMES #MICROENCAPSULATION #PHASE-TRANSITIONS #DELIVERY-SYSTEMS #FERROUS SULFATE #CHEDDAR CHEESE #ESSENTIAL OIL #ENCAPSULATION #PHOSPHATIDYLCHOLINE #BIOAVAILABILITY #FORMULATIONS #CAROTENOIDS #ENGINEERING, CHEMICAL
Tipo

article

original article

publishedVersion