Vitamin E Alters Inflammatory Gene Expression in Alcoholic Chronic Pancreatitis
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
23/10/2013
23/10/2013
2012
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Resumo |
Objective: To evaluate the effect of vitamin E supplementation on pancreatic gene expression of inflammatory markers in rats with alcoholic chronic pancreatitis. Methods: Wistar rats were divided into 3 groups: control (1), alcoholic chronic pancreatitis without (2) and with (3) vitamin E supplementation. Pancreatitis was induced by a liquid diet containing ethanol, cyclosporin A and cerulein. a-tocopherol content in plasma and liver and pancreas histopathology were analyzed. Gene expression of inflammatory biomarkers was analyzed by the quantitative real-time PCR technique. Results: The animals that received vitamin E supplementation had higher alpha-tocopherol amounts in plasma and liver. The pancreas in Group 1 showed normal histology, whereas in Groups 2 and 3, mild to moderate tissue destruction foci and mononuclear cell infiltration were detected. Real-time PCR analysis showed an increased expression of all genes in Groups 2 and 3 compared to Group 1. Vitamin E supplementation decreased the transcript number of 5 genes (alpha-SMA, COX-2, IL-6, MIP-3 alpha and TNF-alpha) and increased the transcript number of 1 gene (Pap). Conclusion: Vitamin E supplementation had anti-inflammatory and beneficial effects on the pancreatic gene expression of some inflammatory biomarkers in rats with alcoholic chronic pancreatitis, confirming its participation in the inflammatory response mechanisms in the pancreas. Copyright (c) 2012 S. Karger AG, Basel FAEPA (Foundation to Support Education, Research and Assistance of the Clinics Hospital of the Faculty of Medicine of Ribeirao Preto, University of Sao Paulo) FAEPA (Foundation to Support Education, Research and Assistance of the Clinics Hospital of the Faculty of Medicine of Ribeirao Preto, University of Sao Paulo) CNPq (National Council for Scientific and Technological Development in Brazil) CNPq (National Council for Scientific and Technological Development in Brazil) |
Identificador |
JOURNAL OF NUTRIGENETICS AND NUTRIGENOMICS, BASEL, v. 5, n. 2, supl. 1, Part 3, pp. 94-105, JUL, 2012 1661-6499 http://www.producao.usp.br/handle/BDPI/35689 10.1159/000336076 |
Idioma(s) |
eng |
Publicador |
KARGER BASEL |
Relação |
JOURNAL OF NUTRIGENETICS AND NUTRIGENOMICS |
Direitos |
closedAccess Copyright KARGER |
Palavras-Chave | #ALCOHOLIC CHRONIC PANCREATITIS #INFLAMMATION #VITAMIN E #QUANTITATIVE REAL-TIME PCR #GENE EXPRESSION #LOW-DENSITY LIPOPROTEINS #LIPID-PEROXIDATION #STELLATE CELLS #ALPHA-TOCOPHEROL #ETHANOL #RAT #LIVER #PROTEIN #INJURY #CYCLOOXYGENASE-2 #GENETICS & HEREDITY #NUTRITION & DIETETICS |
Tipo |
article original article publishedVersion |