Protective effects of bone marrow mononuclear cell therapy on lung and heart in an elastase-induced emphysema model
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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| Data(s) |
04/11/2013
04/11/2013
2012
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| Resumo |
We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and consequently the heart in experimental elastase-induced emphysema. Twenty-four female C57BL/6 mice were intratracheally instilled with saline (C group) or porcine pancreatic elastase (E group) once a week during 4 weeks. C and E groups were randomized into subgroups receiving saline (SAL) or male BMDMCs (2 x 10(6), CELL) intravenously 3 h after the first saline or elastase instillation. Compared to E-SAL group, E-CELL mice showed, at 5 weeks: lower mean linear intercept, neutrophil infiltration, elastolysis, collagen fiber deposition in alveolar septa and pulmonary vessel wall, lung cell apoptosis, right ventricle wall thickness and area, higher endothelial growth factor and insulin-like growth factor mRNA expressions in lung tissue, and reduced platelet-derived growth factor, transforming growth factor-beta, and caspase-3 expressions. In conclusion, BMDMC therapy was effective at modulating the inflammatory and remodeling processes in the present model of elastase-induced emphysema. (c) 2012 Elsevier B.V. All rights reserved. Centres of Excellence Program (PRONEX-FAPERJ) Centres of Excellence Program (PRONEXFAPERJ) Brazilian Council for Scientific and Technological Development (MCT/CNPq) Brazilian Council for Scientific and Technological Development (MCT/CNPq) Carlos Chagas Filho Rio de Janeiro State Research Supporting Foundation (FAPERJ) Carlos Chagas Filho Rio de Janeiro State Research Supporting Foundation (FAPERJ) Sao Paulo State Research Support Foundation (FAPESP) Sao Paulo State Research Support Foundation (FAPESP) National Institute of Science and Technology of Drugs and Medicine (INCTINOFAR) National Institute of Science and Technology of Drugs and Medicine (INCT-INOFAR) Coordination for the Improvement of Higher Level Personnel (CAPES) Coordination for the Improvement of Higher Level Personnel (CAPES) |
| Identificador |
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY, AMSTERDAM, v. 182, n. 1, supl. 1, Part 3, pp. 26-36, 42156, 2012 1569-9048 http://www.producao.usp.br/handle/BDPI/40801 10.1016/j.resp.2012.01.002 |
| Idioma(s) |
eng |
| Publicador |
ELSEVIER SCIENCE BV AMSTERDAM |
| Relação |
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY |
| Direitos |
closedAccess Copyright ELSEVIER SCIENCE BV |
| Palavras-Chave | #ELASTASE #EMPHYSEMA #REMODELING #STEM CELLS #CARDIOVASCULAR FUNCTION #OBSTRUCTIVE PULMONARY-DISEASE #MESENCHYMAL STEM-CELLS #ENDOTHELIAL GROWTH-FACTOR #INJURY #FIBROSIS #REPAIR #TRANSPLANTATION #INFLAMMATION #COPD #MICE #PHYSIOLOGY #RESPIRATORY SYSTEM |
| Tipo |
article original article publishedVersion |
