Plasma corticosterone elevation inhibits the activation of nuclear factor kappa B (NFKB) in the Syrian hamster pineal gland


Autoria(s): Ferreira, Zulma Felisbina da Silva; Bothorel, B.; Markus, Regina Pekelmann; Simonneaux, V.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

30/10/2013

30/10/2013

02/08/2013

Resumo

We evaluated how the mild stress-induced increase in endogenous corticosterone affected the pineal gland in Syrian hamsters (Mesocricetus auratus). The animals were maintained under constant light for 1 day, instead of a cycle of 14:10-h, to increase the circulating corticosterone levels during the daytime. The nuclear translocation of nuclear factor kappa B (NFKB), which is the pivotal transcription factor for stress and injury, presented a daily rhythm in normal animals. NFKB nuclear content increased linearly from the onset of light [Zeitgeber Time 0 (ZT0)] until ZT11 and decreased after ZT12 when the plasma corticosterone peak was detected in normal animals. However, the 24-h profiles of the two curves were different, and they did not clearly support an exclusive relationship between corticosterone levels and NFKB content. Therefore, we tested the effect of increased endogenous corticosterone through inducing mild stress by maintaining daytime illumination for one night. This stressful condition, which increased daytime corticosterone levels, resulted in a daytime decrease in NFKB nuclear content, and this was inhibited by mifepristone. Overall, this study shows that NFKB has a daily rhythm in Syrian hamster pineal glands and, by increasing endogenous corticosterone with a stressful condition, NFKB activity is regulated. Therefore, this study suggests that the pineal gland in the Syrian hamster is a sensor of stressful conditions.

FAPESP [2006/57009-6, 2007/07871-6]

FAPESP

CAPES/COFECUB [519/05]

CAPESCOFECUB

CNPq

CNPq

Identificador

STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS, LONDON, v. 15, n. 3, supl. 1, Part 3, pp. 339-347, MAY, 2012

1025-3890

http://www.producao.usp.br/handle/BDPI/36889

10.3109/10253890.2011.627070

http://dx.doi.org/10.3109/10253890.2011.627070

Idioma(s)

eng

Publicador

INFORMA HEALTHCARE

LONDON

Relação

STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS

Direitos

restrictedAccess

Copyright INFORMA HEALTHCARE

Palavras-Chave #CONSTANT LIGHT #CORTICOSTERONE #MELATONIN #MILD STRESS #NUCLEAR FACTOR KAPPA B #PINEAL GLAND #NITRIC-OXIDE PRODUCTION #MELATONIN SYNTHESIS #ENDOTHELIAL-CELLS #RAT PINEAL #IN-VIVO #TRANSCRIPTION FACTORS #GENE-EXPRESSION #IMMUNE-RESPONSE #TNF-ALPHA #ENDOCRINE #BEHAVIORAL SCIENCES #ENDOCRINOLOGY & METABOLISM #NEUROSCIENCES
Tipo

article

original article

publishedVersion