Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
04/11/2013
04/11/2013
02/08/2013
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Resumo |
The objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in Brazilian fish pacu (Piaractus mesopotamicus), in addition to verifying the influence of beta-glucan as an immunostimulant. Fish were fed with diets containing 0, 0.1 and 1% beta-glucan, during seven days, and then inoculated with Aeromonas hydrophila. Seven days after the challenge, they were bled for serum extraction. The methodology consisted of a kinetic assay that allows calculating the required time for serum proteins of the complement to promote 50% lysis of a rabbit red blood cell suspension. The method developed in mammals was successfully applied for pacu and determined that the hemolytic activity of the proteins of the complement system (alternative pathway) increased after the pathogen challenge, but was not influenced by the beta-glucan treatment. |
Identificador |
REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE, VICOSA, v. 41, n. 2, pp. 237-241, FEB, 2012 1516-3598 http://www.producao.usp.br/handle/BDPI/37757 10.1590/S1516-35982012000200001 |
Idioma(s) |
eng |
Publicador |
REVISTA BRASILEIRA ZOOTECNIA BRAZILIAN JOURNAL ANIMAL SCI VICOSA |
Relação |
REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE |
Direitos |
openAccess Copyright REVISTA BRASILEIRA ZOOTECNIA BRAZILIAN JOURNAL ANIMAL SCI |
Palavras-Chave | #A. HYDROPHILA #BETA-GLUCAN #IMMUNE RESPONSE #PACU #TROUT ONCORHYNCHUS-MYKISS #DIETARY BETA-1,3 GLUCAN #DISEASE RESISTANCE #RAINBOW-TROUT #VITAMIN-C #AEROMONAS-HYDROPHILA #DEFENSE-MECHANISMS #FISH #MACROGARD #RESPONSES #AGRICULTURE, DAIRY & ANIMAL SCIENCE #VETERINARY SCIENCES |
Tipo |
article original article publishedVersion |