Molecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
|---|---|
| Data(s) |
01/11/2013
01/11/2013
2012
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| Resumo |
Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-specific probes targeted to the fungal ITS 1 region. This assay provides a new tool for pathogen identification and for epidemiological, drug resistance and virulence studies of these organisms. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [302992/2005-7] Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) |
| Identificador |
GENETICS AND MOLECULAR BIOLOGY, RIBEIRAO PRETO, v. 35, n. 1, pp. 122-125, JUL-AUG, 2012 1415-4757 http://www.producao.usp.br/handle/BDPI/37374 10.1590/S1415-47572011005000059 |
| Idioma(s) |
eng |
| Publicador |
SOC BRASIL GENETICA RIBEIRAO PRETO |
| Relação |
GENETICS AND MOLECULAR BIOLOGY |
| Direitos |
openAccess Copyright SOC BRASIL GENETICA |
| Palavras-Chave | #CANDIDA PALMIOLEOPHILA #DEBARYOMYCES HANSENII #DIFFERENTIAL IDENTIFICATION #PICHIA GUILLIERMONDII #REAL-TIME PCR #YEAST BIOCHEMICAL CARD #PATHOGEN #ALBICANS #BIOCHEMISTRY & MOLECULAR BIOLOGY #GENETICS & HEREDITY |
| Tipo |
article original article publishedVersion |
