MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE)


Autoria(s): Clivati, Debora; Gitzendanner, Matthew Aaron; Silva Hilsdorf, Alexandre Wagner; Araujo, Welington Luiz; Oliveira de Miranda, Vitor Fernandes
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

29/10/2013

29/10/2013

2012

Resumo

Premise of the study: Microsatellite markers were developed to study the genetic diversity and population structure of the carnivorous bladderwort Utricularia reniformis, which is endemic to the Atlantic Forest of southern and southeastern Brazil. Cross-species amplification was tested in U. gibba, U. neottioides, U. subulata, and Pinguicula benedicta. Methods and Results: The U. reniformis genome was sequenced in a 454 GS FLX sequencer, and eight primer sets were developed based on the microsatellites identified from the reads. All loci are polymorphic, showing 1.6 to 4.8 alleles per population. Preliminary results show that primer sets are suitable for population-level studies. Cross-species amplifi cation was successful in three other Utricularia species and one Pinguicula species. Conclusions: Markers developed in this study provide tools for analyses of intra- and interpopulation genetic diversity in Utricularia and Pinguicula.

Fundacao de Amparo a Pesquisa do Estado da Sao Paulo (FAPESP)

Fundacao de Amparo a Pesquisa do Estado da Sao Paulo (FAPESP) [2008/22239-9, 2007/58277-7]

Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [5316/10-3]

Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)

Identificador

AMERICAN JOURNAL OF BOTANY, ST LOUIS, v. 99, n. 9, supl. 1, Part 3, pp. E375-E378, SEP, 2012

0002-9122

http://www.producao.usp.br/handle/BDPI/36442

10.3732/ajb.1200080

http://dx.doi.org/10.3732/ajb.1200080

Idioma(s)

eng

Publicador

BOTANICAL SOC AMER INC

ST LOUIS

Relação

AMERICAN JOURNAL OF BOTANY

Direitos

restrictedAccess

Copyright BOTANICAL SOC AMER INC

Palavras-Chave #ATLANTIC FOREST #BLADDERWORTS #ENDEMIC PLANTS #GENETIC DIVERSITY #LENTIBULARIACEAE #UTRICULARIA RENIFORMIS #FOREST #LOCI #PLANT SCIENCES
Tipo

article

original article

publishedVersion