Cytometric quantification of singlet oxygen in the human malaria parasite Plasmodium falciparum


Autoria(s): Butzloff, Sabine; Groves, Matthew R.; Wrenger, Carsten; Mueller, Ingrid B.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

01/11/2013

01/11/2013

2012

Resumo

The malaria parasite Plasmodium falciparum proliferates within human erythrocytes and is thereby exposed to a variety of reactive oxygen species (ROS) such as hydrogen peroxide, hydroxyl radical, superoxide anion, and highly reactive singlet oxygen (1O2). While most ROS are already well studied in the malaria parasite, singlet oxygen has been neglected to date. In this study we visualized the generation of 1O2 by live cell fluorescence microscopy using 3-(p-aminophenyl) fluorescein as an indicator dye. While 1O2 is found restrictively in the parasite, its amount varies during erythrocytic schizogony. Since the photosensitizer cercosporin generates defined amounts of 1O2 we have established a new cytometric method that allows the stage specific quantification of 1O2. Therefore, the parasites were first classified into three main stages according to their respective pixel-area of 200600 pixels for rings, 7001,200 pixels for trophozoites and 1,4002,500 pixels for schizonts. Interestingly the highest mean concentration of endogenous 1O2 of 0.34 nM is found in the trophozoites stage, followed by 0.20 nM (ring stage) and 0.10 nM (schizont stage) suggesting that 1O2 derives predominantly from the digestion of hemoglobin. (c) 2012 International Society for Advancement of Cytometry

Identificador

CYTOMETRY PART A, HOBOKEN, v. 81A, n. 8, supl. 1, Part 1, pp. 698-703, AUG, 2012

1552-4922

http://www.producao.usp.br/handle/BDPI/37446

10.1002/cyto.a.22081

http://dx.doi.org/10.1002/cyto.a.22081

Idioma(s)

eng

Publicador

WILEY-BLACKWELL

HOBOKEN

Relação

CYTOMETRY PART A

Direitos

closedAccess

Copyright WILEY-BLACKWELL

Palavras-Chave #PLASMODIUM FALCIPARUM #SINGLET OXYGEN #REACTIVE OXYGEN SPECIES #PIXEL-AREA #CERCOSPORIN #AMINOPHENYL FLUORESCEIN (APF) #VITAMIN-B6 BIOSYNTHESIS #METABOLISM #STRESS #GROWTH #ERYTHROCYTES #CERCOSPORIN #DERIVATIVES #SUPEROXIDE #BLOOD #BIOCHEMICAL RESEARCH METHODS #CELL BIOLOGY
Tipo

article

original article

publishedVersion