Cytotoxicity evaluation of Curcuma zedoaria (Christm.) Roscoe fluid extract used in oral hygiene products


Autoria(s): Fernandes, João Paulo dos Santos; Moura, Anna Carolina Volpi Mello de; Marques, Marcia Martins; Nicoletti, Maria Aparecida
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

29/10/2013

29/10/2013

02/08/2013

Resumo

Objective. This in vitro study evaluated the cytotoxic effects of the Curcuma zedoaria (Christm.) Roscoe (popular name: zedoary) fluid extract, as used in preparations for oral hygiene, mostly for anti-septic purposes. Materials and methods. The cell viability and cell growth were assessed by Trypan blue dye exclusion assay using the LMF cell line derived from oral mucosa. Cell viability (short-term assay) was measured 0, 6, 12 and 24 h after contact with the fluid extract. Cell growth (long-term assay) was analyzed in 1, 3, 5 and 7 days. The experimental groups were those testing the fluid extract obtained from the zedoary rhizome and the extractor liquid (ethanol 70 degrees GL) in the concentrations of 0.01-0.0001% v/v. Fresh DMEM were used in the control cultures. Results. Short-term assay-all studied cultures maintained stable cell viability; Long-term assay-there was progressive cell growth in all studied cultures. Conclusion. According to the results, the zedoary fluid extract presents low cytotoxicity and probably can be used in the oral hygiene products.

FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Identificador

ACTA ODONTOLOGICA SCANDINAVICA, LONDON, v. 70, n. 6, supl. 1, Part 3, pp. 610-614, DEC, 2012

0001-6357

http://www.producao.usp.br/handle/BDPI/36226

10.3109/00016357.2011.645060

http://dx.doi.org/10.3109/00016357.2011.645060

Idioma(s)

eng

Publicador

INFORMA HEALTHCARE

LONDON

Relação

ACTA ODONTOLOGICA SCANDINAVICA

Direitos

restrictedAccess

Copyright INFORMA HEALTHCARE

Palavras-Chave #CELL CULTURE #CYTOTOXICITY #ZEDOARY #VEGETAL EXTRACT #ANTIMICROBIAL ACTIVITY #ANALGESIC PROPERTIES #IN-VITRO #INHIBITION #DENTIFRICE #BRAZIL #DENTISTRY, ORAL SURGERY & MEDICINE
Tipo

article

original article

publishedVersion