Local Injections of Adipose-Derived Mesenchymal Stem Cells Modulate Inflammation and Increase Angiogenesis Ameliorating the Dystrophic Phenotype in Dystrophin-Deficient Skeletal Muscle


Autoria(s): Pinheiro, Carlos Hermano da Justa; Farias de Queiroz, Jean Cesar; Ferreira, Lucas Guimarães; Vitzel, Kaio Fernando; Nachbar, Renato Tadeu; Oliveira de Sousa, Luis Gustavo; Junior, Alcione Lescano de Souza; Nunes, Maria Tereza; Curi, Rui
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

01/11/2013

01/11/2013

02/08/2013

Resumo

The effects of adipose-derived mesenchymal stem cells (ADMSC) transplantation on degeneration, regeneration and skeletal muscle function were investigated in dystrophin-deficient mice (24-week-old). ADMSC transplantation improved muscle strength and, resistance to fatigue. An increase in fiber cross-sectional area and in the number of fibers with centralized nuclei and augment of myogenin content were observed. In ADMSC-treated muscles a decrease in muscle content of TNF-alpha, IL-6 and oxidative stress measured by Amplex(A (R)) reagent were observed. The level of TGF-beta 1 was lowered whereas that of VEGF, IL-10 and IL-4 were increased by ADMSC treatment. An increase in markers of macrophage M1 (CD11 and F4-80) and a decrease in T lymphocyte marker (CD3) and arginase-1 were also observed in ADMSCs-treated dystrophic muscle. No change was observed in iNOS expression. Increased phosphorylation of Akt, p70S6k and 4E-BP1 was found in dystrophic muscles treated with ADMSC. These results suggest that ADMSC transplantation modulates inflammation and improves muscle tissue regeneration, ameliorating the dystrophic phenotype in dystrophin-deficient mice.

Sao Paulo Research Foundation-FAPESP [2008/54693-9]

Sao Paulo Research Foundation (FAPESP)

National Council for Scientific and Technological Development (CNPq) [573557/2008-0]

National Council for Scientific and Technological Development (CNPq)

Identificador

STEM CELL REVIEWS AND REPORTS, TOTOWA, v. 8, n. 2, supl. 1, Part 3, pp. 363-374, JUN, 2012

1550-8943

http://www.producao.usp.br/handle/BDPI/37334

10.1007/s12015-011-9304-0

http://dx.doi.org/10.1007/s12015-011-9304-0

Idioma(s)

eng

Publicador

HUMANA PRESS INC

TOTOWA

Relação

STEM CELL REVIEWS AND REPORTS

Direitos

closedAccess

Copyright HUMANA PRESS INC

Palavras-Chave #MUSCULAR DYSTROPHY #MESENCHYMAL STEM CELLS #STEM CELL TRANSPLANTATION #INFLAMMATION #CYTOKINES #ANGIOGENESIS #SKELETAL MUSCLE FUNCTION #DUCHENNE MUSCULAR-DYSTROPHY #IMPROVES CARDIAC-FUNCTION #BONE-MARROW #STROMAL CELLS #NITRIC-OXIDE #MDX MICE #MYOCARDIAL-INFARCTION #SYSTEMIC DELIVERY #GENE-TRANSFER #RAT MODEL #CELL & TISSUE ENGINEERING #CELL BIOLOGY #MEDICINE, RESEARCH & EXPERIMENTAL
Tipo

article

original article

publishedVersion