Determination of dimethyltryptamine and beta-carbolines (ayahuasca alkaloids) in plasma samples by LC-MS/MS
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
01/11/2013
01/11/2013
2012
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Resumo |
Background: Ayahuasca is a psychoactive plant beverage originally used by indigenous people throughout the Amazon Basin, long before its modern use by syncretic religious groups established in Brazil, the USA and European countries. The objective of this study was to develop a method for quantification of dimethyltryptamine and beta-carbolines in human plasma samples. Results: The analytes were extracted by means of C18 cartridges and injected into LC-MS/MS, operated in positive ion mode and multiple reaction monitoring. The LOQs obtained for all analytes were below 0.5 ng/ml. By using the weighted least squares linear regression, the accuracy of the analytical method was improved at the lower end of the calibration curve (from 0.5 to 100 ng/ml; r(2)> 0.98). Conclusion: The method proved to be simple, rapid and useful to estimate administered doses for further pharmacological and toxicological investigations of ayahuasca exposure. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2006/00388-5] Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) |
Identificador |
BIOANALYSIS, LONDON, v. 4, n. 14, pp. 1731-1738, JUL, 2012 1757-6180 http://www.producao.usp.br/handle/BDPI/37178 10.4155/BIO.12.124 |
Idioma(s) |
eng |
Publicador |
FUTURE SCI LTD LONDON |
Relação |
BIOANALYSIS |
Direitos |
closedAccess Copyright FUTURE SCI LTD |
Palavras-Chave | #HARMALA ALKALOIDS #N,N-DIMETHYLTRYPTAMINE #PHARMACOKINETICS #PHARMACOLOGY #BEVERAGE #HOASCA #BIOCHEMICAL RESEARCH METHODS #CHEMISTRY, MEDICINAL |
Tipo |
article original article publishedVersion |