Purification and characterization of Hb 98-114: A novel hemoglobin-derived antimicrobial peptide from the midgut of Rhipicephalus (Boophilus) microplus


Autoria(s): Belmonte, Rodrigo; Cruz, Carlos Eduardo Silva da; Pires, Jose R.; Daffre, Sirlei
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

30/10/2013

30/10/2013

02/08/2013

Resumo

The antimicrobial activity of hemoglobin fragments (hemocidins) has been reported in a variety of models. The cattle tick Rhipicephalus (Boophilus) microplus is a blood sucking arthropod from where the first in vivo-generated hemocidin was characterized (Hb 33-61). In the present work we identified a novel antimicrobial peptide from the midgut of fully engorged R. (B.) microplus females, which comprises the amino acids 98-114 of the alpha subunit of bovine hemoglobin, and was designated Hb 98-114. This peptide was active against several yeast and filamentous fungi, although no activity was detected against bacteria up to 50 mu M of the synthetic peptide. Hb 98-114 was capable of permeabilizing Candida albicans cell membrane and had a fungicidal effect against this yeast. Circulardichroism (CD) and nuclear magnetic resonance (NMR) experiments showed that Hb 98-114 has a random conformation in aqueous solution but switches to an alpha-helical conformation in the presence of sodium dodecyl sulfate (SDS). This alpha helix adopts an amphipathic structure which may be the mechanism of cell membrane permeabilization. Importantly, Hb 98-114 may play an important role in defending the tick midgut against fungal pathogens and is the first hemocidin with specific antifungal activity to be characterized. (C) 2012 Elsevier Inc. All rights reserved.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ)

Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Coordenacao de Aperfeicoamento Pessol de Nivel Superior (CAPES)

Coordenacao de Aperfeicoamento Pessol de Nivel Superior (CAPES)

Identificador

PEPTIDES, NEW YORK, v. 37, n. 1, supl. 1, Part 3, pp. 120-127, SEP, 2012

0196-9781

http://www.producao.usp.br/handle/BDPI/36961

10.1016/j.peptides.2012.05.017

http://dx.doi.org/10.1016/j.peptides.2012.05.017

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE INC

NEW YORK

Relação

PEPTIDES

Direitos

closedAccess

Copyright ELSEVIER SCIENCE INC

Palavras-Chave #ANTIMICROBIAL PEPTIDES #HEMOGLOBIN DIGESTION #RHIPICEPHALUS (BOOPHILUS) MICROPLUS #ALPHA HELICAL PEPTIDES #MEMBRANE PERMEABILIZATION #BOS-INDICUS CATTLE #L-LIKE ENZYME #BOVINE HEMOGLOBIN #TICK #BLOOD #PROTEINS #PRODUCTIVITY #EXPRESSION #ASSIGNMENT #FRAGMENT #BIOCHEMISTRY & MOLECULAR BIOLOGY #PHARMACOLOGY & PHARMACY
Tipo

article

original article

publishedVersion