mu(1)- and 5-HT1-dependent mechanisms in the anterior pretectal nucleus mediate the antinociceptive effects of retrosplenial cortex stimulation in rats
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
30/10/2013
30/10/2013
02/08/2013
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Resumo |
Aim: This study examines if injection of cobalt chloride (CoCl2) or antagonists of muscarinic cholinergic (atropine), mu(1)-opioid (naloxonazine) or 5-HT1 serotonergic (methiothepin) receptors into the dorsal or ventral portions of the anterior pretectal nucleus (APtN) alters the antinociceptive effects of stimulating the retrosplenial cortex (RSC) in rats. Main method: Changes in the nociceptive threshold were evaluated using the tail flick or incision pain tests in rats that were electrically stimulated at the RSC after the injection of saline, CoCl2 (1 mM, 0.10 mu L) or antagonists into the dorsal or ventral APtN. Key findings: The injection of CoCl2, naloxonazine (5 mu g/0.10 mu L) or methiothepin (3 mu g/0.10 mu L) into the dorsal APtN reduced the stimulation-produced antinociception from the RSC in the rat tail flick test. Reduction of incision pain was observed following stimulation of the RSC after the injection of the same substances into the ventral APtN. The injection of atropine (10 ng/0.10 mu L) or ketanserine (5 mu g/0.10 mu L) into the dorsal or ventral APtN was ineffective against the antinociception resulting from RSC stimulation. Significance: mu(1)-opioid- and 5-HT1-expressing neurons and cell processes in dorsal and ventral APtN are both implicated in the mediation of stimulation-produced antinociception from the RSC in the rat tail flick and incision pain tests, respectively. (c) 2012 Elsevier Inc. All rights reserved. FAPESP FAPESP FAPESP fellowships FAPESP fellowships |
Identificador |
LIFE SCIENCES, OXFORD, v. 90, n. 23-24, supl. 1, Part 3, pp. 950-955, 41791, 2012 0024-3205 http://www.producao.usp.br/handle/BDPI/36747 10.1016/j.lfs.2012.04.023 |
Idioma(s) |
eng |
Publicador |
PERGAMON-ELSEVIER SCIENCE LTD OXFORD |
Relação |
LIFE SCIENCES |
Direitos |
closedAccess Copyright PERGAMON-ELSEVIER SCIENCE LTD |
Palavras-Chave | #ANTINOCICEPTION #RETROSPLENIAL CORTEX #TAIL FLICK TEST #INCISION PAIN #ANTERIOR PRETECTAL NUCLEUS #DORSAL HORN NEURONS #NOXIOUS STIMULI #PAIN #CONNECTIONS #PROJECTIONS #MODULATION #MEDICINE, RESEARCH & EXPERIMENTAL #PHARMACOLOGY & PHARMACY |
Tipo |
article original article publishedVersion |