MIF induces osteoclast differentiation and contributes to progression of periodontal disease in mice
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
29/10/2013
29/10/2013
02/08/2013
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Resumo |
Periodontal disease (PD) is a chronic inflammatory and alveolar bone destructive disease triggered by microorganisms from the oral biofilm. Oral inoculation of mice with the periodontopathogen Aggregatibacter actinomycetemcomitans (Aa) induces marked alveolar bone loss and local production of inflammatory mediators, including Macrophage Migration Inhibitory Factor (MW). The role of MW for alveolar bone resorption during PD is not known. In the present study, experimental PD was induced in BALB/c wild-type mice (WT) and MW knockout mice (MIF-/-) through oral inoculation of Aa. Despite enhanced number of bacteria, MIF-/- mice had reduced infiltration of TRAP-positive cells and reduced alveolar bone loss. This was associated with decreased neutrophil accumulation and increased levels of IL-10 in periodontal tissues. TNF-alpha production was similar in both groups. In vitro, LPS from Aa enhanced osteoclastic activity in a MIF-dependent manner. In conclusion, MIF has role in controlling bacterial growth in the context of PD but contributes more significantly to the progression of bone loss during PD by directly affecting differentiation and activity of osteoclasts. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. CNPq CNPq FAPEMIG FAPEMIG [APQ-4409-4.01-07, PPM-00073-09] |
Identificador |
MICROBES AND INFECTION, AMSTERDAM, v. 14, n. 2, supl. 1, Part 4, pp. 198-206, FEB, 2012 1286-4579 http://www.producao.usp.br/handle/BDPI/36566 10.1016/j.micinf.2011.09.005 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE BV AMSTERDAM |
Relação |
MICROBES AND INFECTION |
Direitos |
closedAccess Copyright ELSEVIER SCIENCE BV |
Palavras-Chave | #AGGREGATIBACTER ACTINOMYCETEMCOMITANS #PERIODONTAL DISEASE #MIF #BONE LOSS #INFLAMMATION #MIGRATION INHIBITORY FACTOR #ACTINOBACILLUS-ACTINOMYCETEMCOMITANS #BONE-RESORPTION #TISSUE DESTRUCTION #RECEPTOR ACTIVATOR #IN-VIVO #MACROPHAGE #CYTOKINE #PATHOGENESIS #EXPRESSION #IMMUNOLOGY #INFECTIOUS DISEASES #MICROBIOLOGY |
Tipo |
article original article publishedVersion |