MIF induces osteoclast differentiation and contributes to progression of periodontal disease in mice


Autoria(s): Moreira Madeira, Mila Fernandes; Queiroz-Junior, Celso Martins; Costa, Graciela Mitre; Santos, Patricia Campi; Silveira, Elcia Maria; Garlet, Gustavo Pompermaier; Cisalpino, Patricia Silva; Teixeira, Mauro Martins; Silva, Tarcilia Aparecida; Souza, Daniele da Gloria
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

29/10/2013

29/10/2013

02/08/2013

Resumo

Periodontal disease (PD) is a chronic inflammatory and alveolar bone destructive disease triggered by microorganisms from the oral biofilm. Oral inoculation of mice with the periodontopathogen Aggregatibacter actinomycetemcomitans (Aa) induces marked alveolar bone loss and local production of inflammatory mediators, including Macrophage Migration Inhibitory Factor (MW). The role of MW for alveolar bone resorption during PD is not known. In the present study, experimental PD was induced in BALB/c wild-type mice (WT) and MW knockout mice (MIF-/-) through oral inoculation of Aa. Despite enhanced number of bacteria, MIF-/- mice had reduced infiltration of TRAP-positive cells and reduced alveolar bone loss. This was associated with decreased neutrophil accumulation and increased levels of IL-10 in periodontal tissues. TNF-alpha production was similar in both groups. In vitro, LPS from Aa enhanced osteoclastic activity in a MIF-dependent manner. In conclusion, MIF has role in controlling bacterial growth in the context of PD but contributes more significantly to the progression of bone loss during PD by directly affecting differentiation and activity of osteoclasts. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

CNPq

CNPq

FAPEMIG

FAPEMIG [APQ-4409-4.01-07, PPM-00073-09]

Identificador

MICROBES AND INFECTION, AMSTERDAM, v. 14, n. 2, supl. 1, Part 4, pp. 198-206, FEB, 2012

1286-4579

http://www.producao.usp.br/handle/BDPI/36566

10.1016/j.micinf.2011.09.005

http://dx.doi.org/10.1016/j.micinf.2011.09.005

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

AMSTERDAM

Relação

MICROBES AND INFECTION

Direitos

closedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #AGGREGATIBACTER ACTINOMYCETEMCOMITANS #PERIODONTAL DISEASE #MIF #BONE LOSS #INFLAMMATION #MIGRATION INHIBITORY FACTOR #ACTINOBACILLUS-ACTINOMYCETEMCOMITANS #BONE-RESORPTION #TISSUE DESTRUCTION #RECEPTOR ACTIVATOR #IN-VIVO #MACROPHAGE #CYTOKINE #PATHOGENESIS #EXPRESSION #IMMUNOLOGY #INFECTIOUS DISEASES #MICROBIOLOGY
Tipo

article

original article

publishedVersion