Differential expression of CD90 and CD14 stem cell markers in malignant breast cancer cell lines


Autoria(s): Lobba, A. R. M.; Forni, M. F.; Carreira, A. C. O.; Sogayar, Mari Cleide
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

29/10/2013

29/10/2013

2012

Resumo

The recently emerged concept of cancer stem cell (CSC) has led to a new hypothesis on the basis for tumor progression. Basically, the CSC theory hypothesizes the presence of a hierarchically organized and relatively rare cell population, which is responsible for tumor initiation, self-renewal, and maintenance, in addition to accumulation of mutation and resistance to chemotherapy. CSCs have recently been described in breast cancer. Different genetic markers have been used to isolate breast CSCs, none of which have been correlated with the tumorigenicity or metastatic potential of the cells, limiting their precise characterization and clinical application in the development of therapeutic protocols. Here, we sought for subpopulations of CSCs by analyzing 10 judiciously chosen stem cell markers in a normal breast cell line (MCF10-A) and in four human breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-435, and Hs578-T) displaying different degrees of metastatic and invasiveness potential. We were able to identify two markers, which are differentially expressed in nontumorigenic versus tumor cells. The CD90 marker was highly expressed in the malignant cell lines. Interestingly, the CD14 molecule displayed higher expression levels in the nontumorigenic cell line. Therefore, we demonstrated that these two markers, which are more commonly used to isolate and characterize stem cells, are differentially expressed in breast tumor cells, when compared with nontumorigenic breast cells. (C) 2012 International Society for Advancement of Cytometry

Fundacao de Apoio a Pesquisa do Estado de Sao Paulo (FAPESP)

Fundacao de Apoio a Pesquisa do Estado de Sao Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Financiadora de Estudos e Projetos (FINEP)

Financiadora de Estudos e Projetos (FINEP)

Banco Nacional de Desenvolvimento Economico e Social (BNDES)

Banco Nacional de Desenvolvimento Economico e Social (BNDES)

Brazilian Ministries of Health (MSDECIT)

Brazilian Ministries of Health (MS-DECIT)

Science and Technology (MCT)

Science and Technology (MCT)

Identificador

CYTOMETRY PART A, HOBOKEN, v. 81A, n. 12, pp. 1084-1091, DEC, 2012

1552-4922

http://www.producao.usp.br/handle/BDPI/36562

10.1002/cyto.a.22220

http://dx.doi.org/10.1002/cyto.a.22220

Idioma(s)

eng

Publicador

WILEY-BLACKWELL

HOBOKEN

Relação

CYTOMETRY PART A

Direitos

closedAccess

Copyright WILEY-BLACKWELL

Palavras-Chave #CD90 #THY-1 #BREAST CANCER #CANCER STEM CELL #CD14 #BREAST CANCER STEM CELL #THY-1 GLYCOPROTEIN #PROGENITOR CELLS #GENE-EXPRESSION #HUMAN LIVER #MOUSE #SUBPOPULATIONS #CD44(+)/CD24(-/LOW) #POPULATION #TUMORS #BIOCHEMICAL RESEARCH METHODS #CELL BIOLOGY
Tipo

article

original article

publishedVersion