Expression of NADPH oxidase in human pancreatic islets
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
|---|---|
| Data(s) |
29/10/2013
29/10/2013
2012
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| Resumo |
Aims: NADPH oxidase (NOX) is a known source of superoxide anions in phagocytic and non-phagocytic cells. In this study, the presence of this enzyme in human pancreatic islets and the importance of NADPH oxidase in human beta-cell function were investigated. Main methods and key findings: In isolated human pancreatic islets, the expression of NADPH oxidase components was evidenced by real-time PCR (p22(PHOX), p47(PHOX) and p67(PHOX)), Western blotting (p47(PHOX) and p67(PHOX)) and immunohistochemistry (p47(PHOX), p67(PHOX) and gp91(PHOX)). Immunohistochemistry experiments showed co-localization of p47(PHOX), p67(PHOX) and gp91(PHOX) (isoform 2 of NADPH oxidase-NOX2) with insulin secreting cells. Inhibition of NADPH oxidase activity impaired glucose metabolism and glucose-stimulated insulin secretion. Significance: These findings demonstrate the presence of the main intrinsic components of NADPH oxidase comprising the NOX2 isoform in human pancreatic islets, whose activity also contributes to human beta-cell function. (C) 2012 Elsevier Inc. All rights reserved. FAPESP FAPESP CNPq CNPq CAPES CAPES FINEP Brazil FINEP Brazil |
| Identificador |
LIFE SCIENCES, OXFORD, v. 91, n. 41493, supl. 1, Part 3, pp. 244-249, SEP 17, 2012 0024-3205 http://www.producao.usp.br/handle/BDPI/36534 10.1016/j.lfs.2012.07.004 |
| Idioma(s) |
eng |
| Publicador |
PERGAMON-ELSEVIER SCIENCE LTD OXFORD |
| Relação |
LIFE SCIENCES |
| Direitos |
closedAccess Copyright PERGAMON-ELSEVIER SCIENCE LTD |
| Palavras-Chave | #HUMAN ISLETS #NADPH OXIDASE #INSULIN SECRETION #BETA-CELLS #GLUCOSE #RAT #GENERATION #PCR #MEDICINE, RESEARCH & EXPERIMENTAL #PHARMACOLOGY & PHARMACY |
| Tipo |
article original article publishedVersion |
