Omega-3 improves glucose tolerance but increases lipid peroxidation and DNA damage in hepatocytes of fructose-fed rats


Autoria(s): Castro, Gabriela Salim Ferreira de; Santos, Raquel Alves dos; Portari, Guilherme Vannucchi; Jordão, Alceu Afonso; Vannucchi, Helio
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

24/10/2013

24/10/2013

01/04/2012

Resumo

The high consumption of fructose is linked to the increase in various characteristics of the metabolic syndrome. Fish oil is beneficial for the treatment of these comorbidities, such as insulin resistance, dyslipidemia, and hepatic steatosis. The objective of this study was to evaluate the consequences of the administration of fish oil concomitant to fructose ingestion during the experiment (45 days) and during the final 15 days in high-fructose-fed rats. Male Wistar rats were divided into 5 groups: control; those receiving 10% fish oil (FO); those receiving 60% fructose (Fr); those receiving 60% fructose and 10% fish oil for 45 days (FrFO); and those receiving fructose plus soybean oil for 30 days and fish oil for the final 15 days of the study (FrFO15). There was an increase in triacylglycerol, serum total cholesterol, and hepatic volume in the Fr group. The FO and FrFO groups experienced an increase in lipid peroxidation and a decrease in serum reduced glutathione. The FrFO group suffered greater hepatic injury, with increased alanine aminotransferase levels and DNA damage. Marked n-3 incorporation occurred in the groups receiving fish oil, favoring a better response to the oral glucose tolerance test. Fructose induced comorbidities of the metabolic syndrome, and the use of fish oil promoted a better glucose tolerance, although it was accompanied by more hepatocyte damage.

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Identificador

APPLIED PHYSIOLOGY NUTRITION AND METABOLISM, OTTAWA, v. 37, n. 2, pp. 233-240, APR, 2012

1715-5312

http://www.producao.usp.br/handle/BDPI/35831

10.1139/H11-154

http://dx.doi.org/10.1139/H11-154

Idioma(s)

eng

Publicador

CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS

OTTAWA

Relação

Applied Physiology, Nutrition, and Metabolism

Direitos

closedAccess

Copyright CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS

Palavras-Chave #POLYUNSATURATED OMEGA-3 FATTY ACIDS #FRUCTOSE #GLUCOSE TOLERANCE #LIPID PEROXIDATION #DNA DAMAGE #LIVER FATTY ACIDS #FATTY-ACIDS #FISH-OIL #INSULIN SENSITIVITY #METABOLIC SYNDROME #DIETARY FRUCTOSE #ALPHA-TOCOPHEROL #LIVER #ANTIOXIDANTS #EXTRACTION #RODENTS #NUTRITION & DIETETICS #PHYSIOLOGY #SPORT SCIENCES
Tipo

article

original article

publishedVersion