Evaluation of Solid-Phase Microextraction using a Polythiophene Film and Liquid Chromatography with Spectrophotometric Detection for the Determination of Antidepressants in Plasma Samples
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
01/10/2013
01/10/2013
2012
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Resumo |
Polythiophene (PTh) phase electropolymerized on the stainless steel wire was evaluated as solid-phase microextraction (SPME), and analysis by liquid chromatography with spectrophotometric detection (LC-UV) for determination of new-generation antidepressants, selective serotonin reuptake inhibitors (SSRIs) (citalopram, paroxetine, fluoxetine and sertraline), in plasma samples. The influence of electropolymerization variables (scan rate, potential range and scan cycles) was evaluated on SPME performance. The SPME variables (extraction time, temperature, matrix pH, ionic strength and desorption procedure), as well as the influence of plasma proteins on sorption mechanisms were also evaluated. The SPME/LC-UV method developed for determination of antidepressants in plasma sample presented a linear range between the limit of quantification (LOQ, 200-250 ng mL(-1)) to 4000 ng mL(-1), and interday precision with coefficient of variation (CV) ranged from 11 to 15%. The proposed method can be a useful tool for the determination of antidepressants in human plasma samples in urgent toxicological analysis after the accidental or suicidal intake of higher doses of medications. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) |
Identificador |
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY, SAO PAULO, v. 23, n. 1, pp. 57-64, JAN, 2012 0103-5053 http://www.producao.usp.br/handle/BDPI/33900 10.1590/S0103-50532012000100009 |
Idioma(s) |
eng |
Publicador |
SOC BRASILEIRA QUIMICA SAO PAULO |
Relação |
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY |
Direitos |
openAccess Copyright SOC BRASILEIRA QUIMICA |
Palavras-Chave | #SOLID-PHASE MICROEXTRACTION #POLYTHIOPHENE #ANTIDEPRESSANTS #LIQUID CHROMATOGRAPHY #MICRO-EXTRACTION #ELECTROCHEMICAL CONTROL #POLYPYRROLE FILMS #SUICIDE #FIBERS #DRUGS #HPLC #CHEMISTRY, MULTIDISCIPLINARY |
Tipo |
article original article publishedVersion |