The involvement of the spleen during chronic phase of Schistosoma mansoni infection in galectin-3(-/-) mice
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
21/10/2013
21/10/2013
2012
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Resumo |
Schistosoma mansoni synthesizes glycoconjugates which interact with galectin-3, eliciting an intense humoral immune response. Moreover, it was demonstrated that galectin-3 regulates B cell differentiation into plasma cells. Splenomegaly is a hallmark event characterized by polyclonal B cell activation and enhancement of antibody production. Here, we investigated whether galectin-3 interferes with spleen organization and B cell compartment during chronic schistosomiasis, using wild type (WT) and galectin-3(-/-) mice. In chronically-infected galectin-3(-/-) mice the histological architecture of the spleen, including white and red pulps, was disturbed with heterogeneous lymphoid follicles, an increased number of plasma cells (CD19(-)B220(-/low)CD138(+)) and a reduced number of macrophages (CD19(-)B220(-)Mac-1(+)CD138(-)) and B lymphocytes (CD19(+)B220(+/high)CD138(-)), compared with the WT infected mice. In the absence of galectin-3 there was an increase of annexin-V+PI- cells and a major presence of apoptotic cells in spleen compared with WT infected mice. In spleen of WT infected mice galectin-3 was largely expressed in lymphoid follicles and extrafollicular sites. Thus, we propose that galectin-3 plays a role in splenic architecture, controlling distinct events such as apoptosis, macrophage activity, B cell differentiation and plasmacytogenesis in the course of S. mansoni infection. FAPERJ (Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro) [E26/110.199/2010] Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ) APABCAM (Associacao TecnicoCientifica Paul Ehrlich) APABCAM (Associacao Tecnico-Cientifica Paul Ehrlich) |
Identificador |
HISTOLOGY AND HISTOPATHOLOGY, MURCIA, v. 27, n. 8, supl., Part 3, pp. 1109-1120, AUG, 2012 0213-3911 |
Idioma(s) |
eng |
Publicador |
F HERNANDEZ MURCIA |
Relação |
HISTOLOGY AND HISTOPATHOLOGY |
Direitos |
restrictedAccess Copyright F HERNANDEZ |
Palavras-Chave | #GALECTIN-3 #SCHISTOSOMA MANSONI #SPLEEN #B-LYMPHOCYTE #MACROPHAGE #EXPERIMENTAL MURINE SCHISTOSOMIASIS #TRYPANOSOMA-CRUZI INFECTION #INFLAMMATORY RESPONSES #DENDRITIC CELLS #MILKY SPOTS #DIFFERENTIATION #MACROPHAGES #EXPRESSION #LACDINAC #PROLIFERATION #CELL BIOLOGY #PATHOLOGY |
Tipo |
article original article publishedVersion |