Calorie restriction increases cerebral mitochondrial respiratory capacity in a NO center dot-mediated mechanism: Impact on neuronal survival
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
21/10/2013
21/10/2013
2012
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Resumo |
Calorie restriction (CR) enhances animal life span and prevents age-related diseases, including neurological decline. Recent evidence suggests that a mechanism involved in CR-induced life-span extension is NO-stimulated mitochondrial biogenesis. We examine here the effects of CR on brain mitochondrial content. CR increased eNOS and nNOS and the content of mitochondria] proteins (cytochrome c oxidase, citrate synthase, and mitofusin) in the brain. Furthermore, we established an in vitro system to study the neurological effects of CR using serum extracted from animals on this diet. In cultured neurons, CR serum enhanced nNOS expression and increased levels of nitrite (a NO product). CR serum also enhanced the levels of cytochrome c oxidase and increased citrate synthase activity and respiratory rates in neurons. CR serum effects were inhibited by L-NAME and mimicked by the NO donor SNAP. Furthermore, both CR sera and SNAP were capable of improving neuronal survival. Overall, our results indicate that CR increases mitochondrial biogenesis in a NO-mediated manner, resulting in enhanced reserve respiratory capacity and improved survival in neurons. (C) 2012 Elsevier Inc. All rights reserved. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo Fundacao de Amparo a Pesquisa do Estado de Sao Paulo Instituto Nacional de Ciencia e Tecnologia de Processos Redox em Biomedicina Instituto Nacional de Ciencia e Tecnologia de Processos Redox em Biomedicina Conselho Nacional de Desenvolvimento Cientifico e Tecnologico Conselho Nacional de Desenvolvimento Cientifico e Tecnologico Coordenacao de Aperfeicoamento do Pessoal de Nivel Superior Coordenacao de Aperfeicoamento do Pessoal de Nivel Superior John Simon Guggenheim Memorial Foundation John Simon Guggenheim Memorial Foundation |
Identificador |
FREE RADICAL BIOLOGY AND MEDICINE, NEW YORK, v. 52, n. 7, p. 1236-1241, APR 1, 2012 0891-5849 http://www.producao.usp.br/handle/BDPI/35172 10.1016/j.freeradbiomed.2012.01.011 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE INC NEW YORK |
Relação |
Free Radical Biology and Medicine |
Direitos |
closedAccess Copyright ELSEVIER SCIENCE INC |
Palavras-Chave | #CALORIC RESTRICTION #NITRIC OXIDE SYNTHASE #MITOCHONDRIAL BIOGENESIS #AGING #ENDOGENOUS NITRIC-OXIDE #SKELETAL-MUSCLE #OXIDATIVE STRESS #CORTICAL-NEURONS #S-NITROSYLATION #COMPLEX-I #BIOGENESIS #GLUTAMATE #BRAIN #MICE #BIOCHEMISTRY & MOLECULAR BIOLOGY #ENDOCRINOLOGY & METABOLISM |
Tipo |
article original article publishedVersion |