A VALIDATED HPLC ANALYTICAL METHOD FOR THE ANALYSIS OF SOLASONINE AND SOLAMARGINE IN IN VITRO SKIN PENETRATION STUDIES


Autoria(s): Tiossi, Renata F. J.; Da Costa, Juliana C.; Miranda, Mariza A.; Praca, Fabiola S. G.; Bentley, Maria Vitoria L. B.; Bastos, Jairo K.; McChesney, James D.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

14/10/2013

14/10/2013

2012

Resumo

To assess topical delivery studies of glycoalkaloids, an analytical method by HPLC-UV was developed and validated for the determination of solasonine (SN) and solamargine (SM) in different skin layers, as well as in a topical formulation. The method was linear within the ranges 0.86 to 990.00 mu g/mL for SN and 1.74 to 1000.00 mu g/mL for SM (r = 0.9996). Moreover, the recoveries for both glycoalkaloids were higher than 88.94 and 93.23% from skin samples and topical formulation, respectively. The method developed is reliable and suitable for topical delivery skin studies and for determining the content of SN and SM in topical formulations.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo- FAPESP [proc. 2007/57538-1]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Identificador

QUIMICA NOVA, SAO PAULO, v. 35, n. 11, pp. 2312-2316, JAN, 2012

0100-4042

http://www.producao.usp.br/handle/BDPI/35052

10.1590/S0100-40422012001100038 

http://dx.doi.org/10.1590/S0100-40422012001100038 

Idioma(s)

eng

Publicador

SOC BRASILEIRA QUIMICA

SAO PAULO

Relação

QUIMICA NOVA

Direitos

openAccess

Copyright SOC BRASILEIRA QUIMICA

Palavras-Chave #SOLASONINE #SOLAMARGINE #VALIDATION #CYCLOSPORINE-A #GLYCOALKALOIDS #APOPTOSIS #EXTRACT #CHEMISTRY, MULTIDISCIPLINARY
Tipo

article

original article

publishedVersion