Genotyping of Human parvovirus B19 among Brazilian patients with hemoglobinopathies


Autoria(s): Slavov, Svetoslav Nanev; Kashima, Simone; Silva-Pinto, Ana Cristina; Covas, Dimas Tadeu
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

14/10/2013

14/10/2013

2012

Resumo

Human parvovirus B19 (B19V) infection can be a life-threatening condition among patients with hereditary (chronic) hemolytic anemias. Our objective was to characterize the infection molecularly among patients with sickle cell disease and thalassemia. Forty-seven patients (37 with sickle cell disease, and 10 with beta-thalassemia major) as well as 47 healthy blood donors were examined for B19V infection by anti-B19V IgG enzyme immunoassay, quantitative PCR, which detects all B19V genotypes, and DNA sequencing. B19V viremia was documented in nine patients (19.1%) as two displayed acute infection and the rest had a low titre viremia (mean 3.4 x 10(4) copies/mL). All donors were negative for B19V DNA. Anti-B19V IgG was detected in 55.3% of the patients and 57.4% among the donors. Based on partial NS1 fragments, all patient isolates were classified as genotype 1 and subgenotype 1A. The evolutionary events of the examined partial NS1 gene sequence were associated with a lack of positive selection. The quantification of all B19V genotypes by a single hydrolytic probe is a technically useful method, but it is difficult to establish relationships between B19V sequence characteristics and infection outcome.

Identificador

CANADIAN JOURNAL OF MICROBIOLOGY, OTTAWA, v. 58, n. 2, supl. 4, Part 1, pp. 200-205, FEB, 2012

0008-4166

http://www.producao.usp.br/handle/BDPI/34507

10.1139/W11-119

http://dx.doi.org/10.1139/W11-119

Idioma(s)

eng

Publicador

CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS

OTTAWA

Relação

CANADIAN JOURNAL OF MICROBIOLOGY

Direitos

restrictedAccess

Copyright CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS

Palavras-Chave #HUMAN PARVOVIRUS B19 #SICKLE CELL DISEASE #THALASSEMIA #REAL-TIME PCR #HUMAN PARVOVIRUS B19 #REAL-TIME PCR #SEQUENCE VARIABILITY #HUMAN ERYTHROVIRUSES #DNA #SEROPREVALENCE #IDENTIFICATION #POPULATION #INFECTION #DIVERSITY #BIOCHEMISTRY & MOLECULAR BIOLOGY #BIOTECHNOLOGY & APPLIED MICROBIOLOGY #IMMUNOLOGY #MICROBIOLOGY
Tipo

article

original article

publishedVersion