Influence of HLA-DRB-1 alleles on the production of antibody against CSP, MSP-1, AMA-1, and DBP in Brazilian individuals naturally infected with Plasmodium vivax

We evaluated the influence of allelic frequency of the human leukocyte antigen (HLA) -DRB1 on the acquisition of antibody response against malaria sporozoite and merozoite peptides in patients with Plasmodium vivax malaria acquired in endemic areas of Brazil. IgG antibodies were detected by enzyme-linked immunosorbent assay against four peptides of circumsporozoite protein (CSP) (amino, carboxyl, and VK210 and VK247 repeats) and peptides of merozoite surface protein 1 (MSP-1), apical membrane antigen 1 (AMA-1), and Duffy-binding protein (DBP). We found an association between HLA-DR3 and HLA-DR5 alleles and lack of antibody response to CSP amino terminal, as well as an association between HILA-DR3 and the highest antibody response to MSP1 (Pv200L). In conclusion, we suggest a potential regulatory role of the H1A-DRB1 alleles in the production of antibodies to a conserved region of P. vivax CSP and MSP1 in Brazilian population exposed to malaria. (C) 2011 Elsevier B.V. All rights reserved.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Sao Paulo State, Brazil [02/09546-1, 06/09546-1]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Sao Paulo State, Brazil

Conselho Nacional para o Desenvolvimento Cientifico e Tecnologico (CNPq), Brasilia, Brazil

Conselho Nacional para o Desenvolvimento Cientifico e Tecnologico (CNPq), Brasilia, Brazil [302353/03-8, 410405/2006-0]

COLCIENCIAS [1106-04-16489)]

Colciencias

Ministry of Social Protection

Ministry of Social Protection [2304-04-19524, 253-2005]

National Institute of Allergy and Infectious Diseases (NIAID) [49486/TMRC]

National Institute of Allergy and Infectious Diseases (NIAID)