Diversification of hAT transposase paralogues in the sugarcane genome


Autoria(s): Jesus, Erika M. de; Ochoa Cruz, Edgar A.; Cruz, Guilherme M. Q.; Van Sluys, Marie-Anne
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

26/09/2013

26/09/2013

2012

Resumo

Transposons are abundant components of eukaryotic genomes, and play important role in genome evolution. The knowledge about these elements should contribute to the understanding of their impact on the host genomes. The hAT transposon superfamily is one of the best characterized superfamilies in diverse organisms, nevertheless, a detailed study of these elements was never carried in sugarcane. To address this question we analyzed 32 cDNAs similar to that of hAT superfamily of transposons previously identified in the sugarcane transcriptome. Our results revealed that these hAT-like transposases cluster in one highly homogeneous and other more heterogeneous lineage. We present evidences that support the hypothesis that the highly homogeneous group is a domesticated transposase while the remainder of the lineages are composed of transposon units. The first is common to grasses, clusters significantly with domesticated transposases from Arabidopsis, rice and sorghum and is expressed in different tissues of two sugarcane cultivars analyzed. In contrast, the more heterogeneous group represents at least two transposon lineages. We recovered five genomic versions of one lineage, characterizing a novel transposon family with conserved DDE motif, named SChAT. These results indicate the presence of at least three distinct lineages of hAT-like transposase paralogues in sugarcane genome, including a novel transposon family described in Saccharum and a domesticated transposase. Taken together, these findings permit to follow the diversification of some hAT transposase paralogues in sugarcane, aggregating knowledge about the co-evolution of transposons and their host genomes.

FAPESP

FAPESP

CNPq

CNPq

Identificador

MOLECULAR GENETICS AND GENOMICS, HEIDELBERG, v. 287, n. 3, pp. 205-219, MAR, 2012

1617-4615

http://www.producao.usp.br/handle/BDPI/33750

10.1007/s00438-011-0670-8

http://dx.doi.org/10.1007/s00438-011-0670-8

Idioma(s)

eng

Publicador

SPRINGER HEIDELBERG

HEIDELBERG

Relação

MOLECULAR GENETICS AND GENOMICS

Direitos

closedAccess

Copyright SPRINGER HEIDELBERG

Palavras-Chave #SUGARCANE #TRANSPOSON #HAT TRANSPOSASE #DOMESTICATED TRANSPOSASE #EUKARYOTIC GENOMES #AC ELEMENT #EVOLUTION #GRASSES #GENES #ARABIDOPSIS #SEQUENCE #COMMON #PLANTS #MAIZE #BIOCHEMISTRY & MOLECULAR BIOLOGY #GENETICS & HEREDITY
Tipo

article

original article

publishedVersion