Physiological effects of glyphosate over amino acid profile in conventional and transgenic soybean (Glycine max)

This paper compares the responses of conventional and transgenic soybean to glyphosate application in terms of the contents of 17 detectable soluble amino acids in leaves, analyzed by HPLC and fluorescence detection. Glutamate, histidine, asparagine, arginine + alanine, glycine + threonine and isoleucine increased in conventional soybean leaves when compared to transgenic soybean leaves, whereas for other amino acids, no significant differences were recorded. Univariate analysis allowed us to make an approximate differentiation between conventional and transgenic lines, observing the changes of some variables by glyphosate application. In addition, by means of the multivariate analysis, using principal components analysis (PCA), cluster analysis (CA) and linear discriminant analysis (LDA) it was possible to identify and discriminate different groups based on the soybean genetic origin. (C) 2011 Elsevier Inc. All rights reserved.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Fundacao de Amparo A Pesquisa do Estado de Sao Paulo (FAPESP) [04/16039-4, 09/54110-6]

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq-Brazil)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil

Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)

Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)

Universidad Nacional de La Pampa

Universidad Nacional de La Pampa

Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES-Brazil)

Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Brazil

Secretaria de Politicas Universitarias (SPU-Argentina)

Secretaria de Politicas Universitarias (SPUArgentina)