Sunflower Oil Supplementation Has Proinflammatory Effects and Does Not Reverse Insulin Resistance in Obesity Induced by High-Fat Diet in C57BL/6Mice

High consumption of polyunsaturated fatty acids, such as sunflower oil has been associated to beneficial effects in plasma lipid profile, but its role on inflammation and insulin resistance is not fully elucidated yet. We evaluated the effect of sunflower oil supplementation on inflammatory state and insulin resistance condition in HFD-induced obese mice. C57BL/ 6 male mice (8 weeks) were divided in four groups: (a) control diet (CD), (b) HFD, (c) CD supplemented with n-6 (CD + n-6), and (d) HFD supplemented with n-6 (HFD + n-6). CD + n-6 and HFD + n-6 were supplemented with sunflower oil by oral gavage at 2 g/ Kg of body weight, three times per week. CD and HFD were supplemented with water instead at the same dose. HFD induced whole andmuscle-specific insulin resistance associated with increased inflammatory markers in insulin-sensitive tissues andmacrophage cells. Sunflower oil supplementation was not efficient in preventing or reducing these parameters. In addition, the supplementation increased pro-inflammatory cytokine production by macrophages and tissues. Lipid profile, on the other hand, was improved with the sunflower oil supplementation in animals fed HFD. In conclusion, sunflower oil supplementation improves lipid profile, but it does not prevent or attenuate insulin resistance and inflammation induced by HFD in C57BL/ 6 mice.

Sao Paulo Research Foundation (FAPESP)

Sao Paulo Research Foundation (FAPESP)

Coordination for the Improvement of Higher Level Personnel (CAPES)

Coordination for the Improvement of Higher Level Personnel (CAPES)

National Council for Scientific and Technological Development (CNPq)/National Institute of Science and Technology in Obesity and Diabetes (INOD)

National Council for Scientific and Technological Development (CNPq)/National Institute of Science and Technology in Obesity and Diabetes (INOD)

Center of Lipid Research and Education (CLEaR)

Center of Lipid Research and Education (CLEaR)

Dean's Office for Research/University of Sao Paulo

Deans Office for Research/University of Sao Paulo