Bone marrow cells of swine: collection and separation


Autoria(s): Branco, Erika; Cabral, Rosa; Gomes, Bruno Duarte; Kfoury Junior, José Roberto; Miglino, Maria Angelica
Contribuinte(s)

Universidade de São Paulo

Data(s)

30/09/2013

30/09/2013

2012

Resumo

Bone marrow is a source of stem cells for greater and easier access, which is widely studied as a provider of hematopoietic and mesenchymal cells for various purposes, mainly therapeutic by the advances in research involving cell therapy. The swine is an animal species commonly used in the pursuit of development of experimental models. Thus, this study aimed to standardize protocol for collection and separation of bone marrow in swines, since this species is widely used as experimental models for various diseases. Twelve animals were used, which underwent bone marrow puncture with access from the iliac crest and cell separation by density gradient followed by a viability test with an average of 98% of viable cells. Given our results, we can ensure the swine as an excellent model for obtaining and isolation of mononuclear cells from bone marrow, stimulating several studies addressing the field of cell therapy. Microsc. Res. Tech., 2012. (C) 2012 Wiley Periodicals, Inc.

Research Support Foundation of Sao Paulo (FAPESP) [05/50081-0]

Identificador

Microscopy Research and Technique, Hoboken, v. 75, n. 7, pp. 917-920, jul, 2012

1059-910X

http://www.producao.usp.br/handle/BDPI/33835

10.1002/jemt.22013

http://dx.doi.org/10.1002/jemt.22013

Idioma(s)

eng

Publicador

Wiley-Blackwell

Hoboken

Relação

Microscopy Research and Technique

Direitos

restrictedAccess

Copyright Wiley-Blackwell

Palavras-Chave #Bone marrow #Stem cells #Swine #STEM CELLS #DOGS #TRANSPLANTATION #PLASTICITY #HARVEST #REPAIR #TISSUE #CÉLULAS-TRONCO #MODELOS ANIMAIS #MEDULA ÓSSEA DE ANIMAL #SUÍNOS #ANATOMY & MORPHOLOGY #BIOLOGY #MICROSCOPY
Tipo

article

original article

publishedVersion