Bone marrow cells of swine: collection and separation
Contribuinte(s) |
Universidade de São Paulo |
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Data(s) |
30/09/2013
30/09/2013
2012
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Resumo |
Bone marrow is a source of stem cells for greater and easier access, which is widely studied as a provider of hematopoietic and mesenchymal cells for various purposes, mainly therapeutic by the advances in research involving cell therapy. The swine is an animal species commonly used in the pursuit of development of experimental models. Thus, this study aimed to standardize protocol for collection and separation of bone marrow in swines, since this species is widely used as experimental models for various diseases. Twelve animals were used, which underwent bone marrow puncture with access from the iliac crest and cell separation by density gradient followed by a viability test with an average of 98% of viable cells. Given our results, we can ensure the swine as an excellent model for obtaining and isolation of mononuclear cells from bone marrow, stimulating several studies addressing the field of cell therapy. Microsc. Res. Tech., 2012. (C) 2012 Wiley Periodicals, Inc. Research Support Foundation of Sao Paulo (FAPESP) [05/50081-0] |
Identificador |
Microscopy Research and Technique, Hoboken, v. 75, n. 7, pp. 917-920, jul, 2012 1059-910X http://www.producao.usp.br/handle/BDPI/33835 10.1002/jemt.22013 |
Idioma(s) |
eng |
Publicador |
Wiley-Blackwell Hoboken |
Relação |
Microscopy Research and Technique |
Direitos |
restrictedAccess Copyright Wiley-Blackwell |
Palavras-Chave | #Bone marrow #Stem cells #Swine #STEM CELLS #DOGS #TRANSPLANTATION #PLASTICITY #HARVEST #REPAIR #TISSUE #CÉLULAS-TRONCO #MODELOS ANIMAIS #MEDULA ÓSSEA DE ANIMAL #SUÍNOS #ANATOMY & MORPHOLOGY #BIOLOGY #MICROSCOPY |
Tipo |
article original article publishedVersion |