Comparison of Brucella agar, CITA and Farrel media for selective isolation of Brucella abortus from semen of bovine bulls
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
01/04/2016
01/04/2016
2015
|
Resumo |
Brucellosis remains as a public health concern worldwide. In domestic animals, the disease is characterized by reproductive disorders in male and female. Besides extensive use of serological tests and recent development of molecular biology techniques, microbiological culture of Brucella species is yet considered a “gold standard” method for diagnosis. Here, semen of 335 bovine bulls was subjected simultaneously to microbiological culture in Brucella agar, Farrell media, and CITA media to evaluate comparatively the best selective media for isolation of Brucella sp. Among all 335 samples, B. abortus B19 strain was isolated from semen of five (1.49%) bulls using the three selective media. However, Farrell media was considered the best selective media for microbiological diagnosis, because of allowed isolation of B. abortus B19 strain from bull semen without bacterial commensal or fungal contamination of plates. |
Formato |
617-620 |
Identificador |
http://dx.doi.org/10.5897/AJMR2014.7252 African Journal of Microbiology Research, v. 9, n. 9, p. 617-620, 2015. 1996-0808 http://hdl.handle.net/11449/137046 10.5897/AJMR2014.7252 2209124317273797 4713326619479934 6968547626233995 5331848159935529 0475227077170072 |
Idioma(s) |
eng |
Relação |
African Journal of Microbiology Research |
Direitos |
closedAccess |
Palavras-Chave | #Brucella agar #Farrell media #CITA media #Bovine bulls #Semen |
Tipo |
info:eu-repo/semantics/article |