Artifactual oxidation of cholesterol during the analysis of cholesterol oxidation products: protective effect of antioxidants


Autoria(s): Guardiola, Francesc; Garcia-Cruset, Sandra; Bou, Ricard; Codony, Rafael
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

07/12/2015

07/12/2015

2004

Resumo

To study the influence of the addition of various antioxidants and their combinations on the artifactual oxidation of cholesterol during analysis, 2 factorial experiments were performed in duplicate. In the first experiment, 2 amounts of the following antioxidants were assayed: ethylenediaminetetraacetic acid (EDTA) disodium salt (0 and 1 mg), pyrogallol (0 and 600 microg), and butylated hydroxytoluene (BHT; 0 and 600 microg); in the second, EDTA disodium salt (0 and 1 mg), ascorbyl palmitate (0 and 600 microg), and BHT (0 and 600 microg). Under low oxidative conditions of dim light, evaporation of solvents at low temperatures, and cold saponification in darkness under nitrogen atmosphere, the addition of antioxidants showed no further protective effect. Furthermore, the presence of ascorbyl palmitate significantly increased the formation of cholesterol-5beta,6beta-epoxide, and 7beta-hydroxycholesterol.

Formato

493-498

Identificador

http://www.ingentaconnect.com/content/aoac/jaoac/2004/00000087/00000002/art00024?token=0052150b425a76e5865462431426f57207921586b25256a5e4e26634a492f2530332976cff5836ed9a

Journal Of Aoac International, v. 87, n. 2, p. 493-498, 2004.

1060-3271

http://hdl.handle.net/11449/130854

15164846

Idioma(s)

eng

Publicador

Journal Of Aoac International

Relação

Journal Of Aoac International

Direitos

closedAccess

Tipo

info:eu-repo/semantics/article