Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/01/2008
|
Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Actin-encoding cDNAs of Nile tilapia (Oreochromis niloticus) were isolated by RT-PCR using total RNA samples of different tissues and further characterized by nucleotide sequencing and in silico amino acid (aa) sequence analysis. Comparisons among the actin gene sequences of O. niloticus and those of other species evidenced that the isolated genes present a high similarity to other fish and other vertebrate actin genes. The highest nucleotide resemblance was observed between O. niloticus and O. mossambicus a-actin and b-actin genes. Analysis of the predicted aa sequences revealed two distinct types of cytoplasmic actins, one cardiac muscle actin type and one skeletal muscle actin type that were expressed in different tissues of Nile tilapia. The evolutionary relationships between the Nile tilapia actin genes and diverse other organisms is discussed. |
Formato |
325-356 |
Identificador |
http://dx.doi.org/10.1590/S1415-47572008000200031 Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 1, p. 325-356, 2008. 1415-4757 http://hdl.handle.net/11449/130606 10.1590/S1415-47572008000200031 S1415-47572008000200031 WOS:000257238500031 S1415-47572008000200031.pdf |
Idioma(s) |
eng |
Publicador |
Sociedade Brasileira de Genética |
Relação |
Genetics and Molecular Biology |
Direitos |
openAccess |
Palavras-Chave | #actin #expression pattern #Nile tilapia #Oreochromis niloticus |
Tipo |
info:eu-repo/semantics/article |