Antifungals and acetylcholinesterase inhibitors from the stem bark of Croton heliotropiifolius


Autoria(s): Queiroz, Marcos Marçal Ferreira; Queiroz, Emerson Ferreira de; Zeraik, Maria Luiza; Marti, G.; Favre-Godal, Q.; Simoes-Pires, C.; Marcourt, Laurence; Carrupt, P. A.; Cuendet, Muriel; Paulo, M. Q.; Bolzani, Vanderlan da Silva; Wolfender, Jean-Luc
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

03/11/2015

03/11/2015

01/12/2014

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

The ethanolic extract of the stem bark of Croton heliotropiifolius Kunth (Euphorbiaceae) showed significant in vitro inhibition of acetylcholinesterase using a dilution spectrophotometric assay and antifungal activity against Candida albicans with a thin layer chromatography (TLC) bioautographic assay. In order to isolate the active compounds, bioassay-guided fractionation was undertaken using HPLC to localize the active compounds. Different zones of the HPLC-UV chromatogram were linked to acetylcholinesterase inhibition or to antifungal activities. In parallel to this HPLC-based activity profiling, HPLC-PDA-ESI-MS and HPLC-TOF-HRMS were used for the early identification of some of the compounds present. The targeted isolation of the active compounds was performed by medium pressure liquid chromatography (MPLC-UV) and further semi-preparative HPLC. Using this approach, nine compounds were isolated, one of them being a new indole alkaloid derivative. The structures of the isolated compounds were elucidated by spectroscopic methods including UV, NMR, MS and HRMS. (C) 2014 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.

Formato

LXXXVIII-xciii

Identificador

http://www.sciencedirect.com/science/article/pii/S1874390014001499

Phytochemistry Letters. Amsterdam: Elsevier Science Bv, v. 10, p. LXXXVIII-xciii, 2014.

1874-3900

http://hdl.handle.net/11449/130317

http://dx.doi.org/10.1016/j.phytol.2014.08.013

WOS:000347915800010

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Phytochemistry Letters

Direitos

closedAccess

Palavras-Chave #Croton heliotropiifolius #HPLC-PDA-ESI-MS #HPLC-TOF-HRMS #Acetylcholinesterase inhibition #Antifungal #Candida albicans
Tipo

info:eu-repo/semantics/article