A comparison of three different methodologies for evaluating Rhipicephalus (Boophilus) rnicroplus susceptibility to topical spray compounds


Autoria(s): Correa, Rafael Rodrigues; Zanetti Lopes, Welber Daniel; Pires Teixeira, Weslen Fabricio; Cruz, Breno Cayeiro; Costa Gomes, Lucas Vinicius; Felippelli, Gustavo; Maciel, Willian Giquelin; Favero, Flavia Carolina; Buzzulini, Carolina; Bichuette, Murilo Abud; Soares, Vando Edesio; Costa, Alvimar Jose da
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

22/10/2015

22/10/2015

15/01/2015

Resumo

This study aimed to compare three different methodologies (Adult Immersion Tests, field trials with naturally infected animals, and a Stall Test using artificially infested cattle) to evaluate the efficacy of two topical formulations that we administered as whole body sprays (15% Cypermethrin + 30% Chlorpyriphos + 15% Fenthion-Colosso (R) FC 30, Ouro Fino Agronegocios; and 60% Dichlorvos + 20% Chlorpyriphos-Ectofos (R), Vallee Saude Animal Ltd.), against a susceptible strain of Rhipicephalus (Boophilus) microplus. To achieve this objective, two natural infestation trials were conducted, as well as two artificial infestation trials (Stall Tests) and two Adult Immersion Tests (AIT). The AIT results showed that both spray formulations achieved 100% efficacy against R. (B.) micro plus fully engorged females. However, when observing results obtained by field trials (natural infestations) and Stall Tests, none of these topically applied compounds reached 100% efficacy or affected the reproductive capacity of the fully engorged female ticks. Additional studies must be conducted to compare these in vivo methodologies with different in vitro techniques, such as the Larval Packet Test. However, based on results obtained here, we can conclude that depending on the spray formulations used, the AIT can overestimate acaricidal efficacy and values of reproductive efficiency of such compounds against R (B.) micro plus. Specifically, when dealing with spray formulations in the Stall Tests, the period of residual action can increase because these animals are sheltered from contact with environmental factors that might interfere with the efficacy of the products tested. It may be necessary to take in vivo trial results into consideration (such as field trials with naturally infested animals or Stall Tests) to standardize a specific in vitro assay, such as the Adult Immersion Test. (C) 2014 Elsevier B.V. All rights reserved.

Formato

115-124

Identificador

http://www.sciencedirect.com/science/article/pii/S0304401714005639

Veterinary Parasitology. Amsterdam: Elsevier Science Bv, v. 207, n. 1-2, p. 115-124, 2015.

0304-4017

http://hdl.handle.net/11449/129631

http://dx.doi.org/10.1016/j.vetpar.2014.11.001

WOS:000348949200015

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Veterinary Parasitology

Direitos

openAccess

Palavras-Chave #Adult immersion test #Artificial infestations #Natural infestation #Spray formulations #Stall test
Tipo

info:eu-repo/semantics/article