TRPV4 activates autonomic and behavioural warmth-defence responses in Wistar rats


Autoria(s): Vizin, Robson Cristiano Lillo; Scarpellini, Carolina da S.; Ishikawa, Débora T.; Correa, Gabriela M.; Souza, Cristiane Oliveira de; Batalhão, Luciane Helena Gargaglioni; Carrettiero, Daniel C.; Bicego, Kênia Cardoso; Almeida, Maria Camila
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

21/10/2015

21/10/2015

01/06/2015

Resumo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Processo FAPESP: 2011/06528-1

Processo FAPESP: 2012/19966-0

Processo FAPESP: 2009/11446-4

Processo FAPESP: 2012/11836-0

Processo FAPESP: 2011/19131-2

Aim In this study, we aimed at investigating the involvement of the warmth-sensitive channel - TRPV4 (in vitro sensitive to temperatures in the range of approx. 24-34 degrees C) - on the thermoregulatory mechanisms in rats.MethodsWe treated rats with a chemical selective agonist (RN-1747) and two antagonists (RN-1734 and HC-067047) of the TRPV4 channel and measured core body temperature, metabolism, heat loss index and preferred ambient temperature.ResultsOur data revealed that chemical activation of TRPV4 channels by topical application of RN-1747 on the skin leads to hypothermia and this effect was blocked by the pre-treatment with the selective antagonist of this channel. Intracerebroventricular treatment with RN-1747 did not cause hypothermia, indicating that the observed response was indeed due to activation of TRPV4 channels in the periphery. Intravenous blockade of this channel with HC-067047 caused an increase in core body temperature at ambient temperature of 26 and 30 degrees C, but not at 22 and 32 degrees C. At 26 degrees C, HC-067047-induced hyperthermia was accompanied by increase in oxygen consumption (an index of thermogenesis), while chemical stimulation of TRPV4 increased tail heat loss, indicating that these two autonomic thermoeffectors in the rat are modulated through TRPV4 channels. Furthermore, rats chemically stimulated with TRPV4 agonist choose colder ambient temperatures and cold-seeking behaviour after thermal stimulation (28-31 degrees C) was inhibited by TRPV4 antagonist.ConclusionOur results suggest, for the first time, that TRPV4 channel is involved in the recruitment of behavioural and autonomic warmth-defence responses to regulate core body temperature.

Formato

275-289

Identificador

http://onlinelibrary.wiley.com/doi/10.1111/apha.12477/abstract;jsessionid=78335D14D0386D15F76F5B56FF0BFCAA.f02t04

Acta Physiologica. Hoboken: Wiley-blackwell, v. 214, n. 2, p. 275-289, 2015.

1748-1708

http://hdl.handle.net/11449/129130

http://dx.doi.org/10.1111/apha.12477

WOS:000354389100015

Idioma(s)

eng

Publicador

Wiley-Blackwell

Relação

Acta Physiologica

Direitos

closedAccess

Palavras-Chave #Tail vasomotor tone #Thermogenesis #Thermopreferendum #Thermoregulation #TRPV4 manipulation #Warmth sensor
Tipo

info:eu-repo/semantics/article