A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
| Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
|---|---|
| Data(s) |
21/10/2015
21/10/2015
01/05/2015
|
| Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects. |
| Formato |
113-116 |
| Identificador |
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015. 1517-8382 http://hdl.handle.net/11449/128702 http://dx.doi.org/10.1590/S1517-838246120130851 S1517-83822015000100113 WOS:000355769900013 S1517-83822015000100113.pdf |
| Idioma(s) |
eng |
| Publicador |
Soc Brasileira Microbiologia |
| Relação |
Brazilian Journal Of Microbiology |
| Direitos |
openAccess |
| Palavras-Chave | #Nitrile hydratase #Amidase #Nitrilase #Amidase inhibitor #High-throughput screening |
| Tipo |
info:eu-repo/semantics/article |
