Biochemical and genotoxic effects of a commercial formulation of the herbicide tebuthiuron in Oreochromis niloticus of different sizes
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
---|---|
Data(s) |
27/04/2015
27/04/2015
2014
|
Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Processo FAPESP: 2010/04028-9 Pesticides are serious contaminants because they are designed to eliminate pests, but they also affect non-target species. The present study aimed to evaluate the biochemical and genetic effects of the herbicide tebuthiuron in Oreochromis niloticus of different sizes. Thus, we analyzed biomarkers in small and large O. niloticus specimens exposed to 62.5, 125 and 250 mg L-1 of tebuthiuron for 72 hours. Fish exposed to 250 mg L-1 had high mortality rates; therefore, the data could not be used. The results showed an increase in EROD activity in fish exposed to 125 mg L-1, but no GST alteration. Antioxidant enzymes GPx and CAT were altered only in the liver of treated fish compared to the control group: CAT decreased in large fish, and GPx increased in small fish. The MDA analysis did not evidence lipid peroxidation. High DNA damage in exposed small fish (not in large fish) was observed using comet assay, but a micronucleus test did not show mutagenicity. Moreover, a comparison between control groups with specimens of different sizes revealed that small fish are more susceptible than large fish to the tebuthiuron effects, since increased comet scores was observed only for smaller fish. |
Formato |
59-67 |
Identificador |
http://www6.univali.br/seer/index.php/eec/article/view/5779 Ecotoxicology and Environmental Contamination, v. 9, n. 1, p. 59-67, 2014. 2317-9643 http://hdl.handle.net/11449/122667 http://dx.doi.org/10.5132/eec.2014.01.008 ISSN2317-9643-2014-09-01-59-67.pdf 6713400866382255 |
Idioma(s) |
eng |
Relação |
Ecotoxicology and Environmental Contamination |
Direitos |
openAccess |
Palavras-Chave | #aquatic toxicology #biomarkers #ecotoxicology #fish #pesticide |
Tipo |
info:eu-repo/semantics/article |