Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture


Autoria(s): Mauro, M. O.; Pesarini, J. R.; Marin-Morales, M. A.; Monreal, M. T. F. D.; Monreal, A. C. D.; Mantovani, M. S.; Oliveira, R. J.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

18/03/2015

18/03/2015

01/01/2014

Resumo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

This study evaluated the mutagenicity and antimutagenicity of inulin in a chromosomal aberration assay in cultures of the meristematic cells of Allium cepa. The treatments evaluated were as follows: negative control - seed germination in distilled water; positive control - aqueous solution of methyl methanesulfonate (10 mu g/mL MMS); mutagenicity - aqueous solutions of inulin (0.015, 0.15, and 1.50 mu g/mL); and antimutagenicity - associations between MMS and the different inulin concentrations. The antimutagenicity protocols established were pretreatment, simultaneous simple, simultaneous with pre-incubation, and post-treatment. The damage reduction percentage (DR%) was 43.56, 27.77, and 55.92% for the pre-treatment; -31.11, 18.51, and 7.03% for the simultaneous simple; 30.43, 19.12, and 21.11% for the simultaneous with pre-incubation; and 64.07, 42.96, and 53.70% for the post-treatment. The results indicated that the most effective treatment for inhibiting damages caused by MMS was the post-treatment, which was followed by the pretreatment, suggesting activity by bioantimutagenesis and desmutagenesis. The Allium cepa assay was demonstrated to be a good screening test for this type of activity because it is easy to perform, has a low cost, and shows DR% that is comparable to that reported studies that evaluated the prevention of DNA damage in mammals by inulin.

Formato

4808-4819

Identificador

http://dx.doi.org/10.4238/2014.February.14.14

Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 13, n. 3, p. 4808-4819, 2014.

1676-5680

http://hdl.handle.net/11449/117560

10.4238/2014.February.14.14

WOS:000340383600011

WOS000340383600011.pdf

Idioma(s)

eng

Publicador

Funpec-editora

Relação

Genetics And Molecular Research

Direitos

openAccess

Palavras-Chave #Inulin #Fiber #Antimutagenic activity
Tipo

info:eu-repo/semantics/article