Association of matrix metalloproteinase inducer (EMMPRIN) with the expression of matrix metalloproteinases-1, -2 and -9 during periapical lesion development
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
18/03/2015
18/03/2015
01/09/2014
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Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Processo FAPESP: 08/58064-6 Processo FAPESP: 11/19511-0 Objective: To evaluate the expression of matrix metalloproteinase inducer (EMMPRIN) and its correlation with the expression of matrix metalloproteinases (MMPs)-1, -2 and -9 during the development of periapical lesion in mice.Methods: Periapical lesions were induced in the lower first molars of mice and after 7, 14, 21 and 42 days the mandibles were removed. The periapical lesions were measured by micro-computed tomography. The expression of EMMPRIN, MMPs-1, -2, and -9 genes were determined by real-time RT-PCR. The location and expression of EMMPRIN and MMPs were evaluated by immunohistochemistry.Results: At 14 days, the periapical lesion area was higher than at 7 days. At 21 and 42 days no statistically significant bone loss was observed in comparison to 14 days. The control group showed discrete and occasional EMMPRIM, MMP-1, -2 and -9 immunostaining in the periodontal ligament fibroblasts. At 7, 14, 21 and 42 days intense immunoexpression was observed for EMMPRIN, MMPs-1, -2 and -9 in the region adjacent to the apical foramen. The EMMPRIN immunoexpression was higher at 7, 14, 21 and 42 days compared with the control. There was a positive correlation between gene expression of EMMPRIN and MMPs in the active phase of periapical lesion development.Conclusion: There is a high expression of EMMPRIM mainly by the inflammatory infiltrate in the region adjacent to the apical foramen during periapical lesion development. Furthermore, the positive correlation with MMP-1, -2, and -9 during the first days after periapical lesion induction indicates that EMMPRIM may be involved in the active phase of periapical lesions development. (C) 2014 Elsevier Ltd. All rights reserved. |
Formato |
944-953 |
Identificador |
http://dx.doi.org/10.1016/j.archoralbio.2014.05.021 Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 59, n. 9, p. 944-953, 2014. 0003-9969 http://hdl.handle.net/11449/116436 10.1016/j.archoralbio.2014.05.021 WOS:000341481600010 |
Idioma(s) |
eng |
Publicador |
Elsevier B.V. |
Relação |
Archives Of Oral Biology |
Direitos |
closedAccess |
Palavras-Chave | #Endodontics #Periapical lesion #Matrix metalloproteinases #EMMPRIN |
Tipo |
info:eu-repo/semantics/article |