Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
| Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
|---|---|
| Data(s) |
02/02/2015
02/02/2015
01/09/2014
|
| Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species. |
| Formato |
841-843 |
| Identificador |
http://dx.doi.org/10.1590/S1517-83822014000300012 Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 45, n. 3, p. 841-843, 2014. 1517-8382 http://hdl.handle.net/11449/114347 10.1590/S1517-83822014000300012 S1517-83822014000300012 S1517-83822014000300012.pdf |
| Idioma(s) |
eng |
| Publicador |
Sociedade Brasileira de Microbiologia |
| Relação |
Brazilian Journal of Microbiology |
| Direitos |
openAccess |
| Palavras-Chave | #bovine tuberculosis #tuberculosis #PCR-multiplex #diagnosis |
| Tipo |
info:eu-repo/semantics/article |
