Deregulation of Annexin-A1 and Galectin-1 Expression in Precancerous Gastric Lesions: Intestinal Metaplasia and Gastric Ulcer


Autoria(s): Teixeira Rossi, Ana Flavia; Duarte, Marcia Cristina; Poltronieri, Ayla Blanco; Valsechi, Marina Curado; Jorge, Yvana Cristina; Neto, Dalisio de-Santi; Rahal, Paula; Oliani, Sonia Maria; Silva, Ana Elizabete
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

03/12/2014

03/12/2014

01/01/2014

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Processo FAPESP: 11/11550-3

Processo FAPESP: 12/15036-8

Objective. Annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) are mediators that play an important role in the inflammatory response and are also associated with carcinogenesis. We investigated mRNA and protein expression in precancerous gastric lesions that participate in the progression cascade to gastric cancer, such as intestinal metaplasia (IM) and gastric ulcer (GU). Methods. Quantitative real-time PCR (qPCR) and immunohistochemical techniques were used to analyze the relative quantification levels (RQ) of ANXA1 and LGALS1 mRNA and protein expression, respectively. Results. Increased relative expression levels of ANXA1 were found in 100% of cases, both in IM (mean RQ = 6.22 +/- 0.06) and in GU (mean RQ = 6.69 +/- 0.10). However, the LGALS1 presented basal expression in both groups (IM: mean RQ = 0.35 +/- 0.07; GU: mean RQ = 0.69 +/- 0.09). Immunohistochemistry revealed significant positive staining for both the AnxA1 and Gal-1 proteins in the epithelial nucleus and cytoplasm as well as in the stroma of the IM and GU groups (P < 0.05) but absence or low immunorectivity in normal mucosa. Conclusion. Our results bring an important contribution by evidencing that both the AnxA1 and Gal-1 anti-inflammatory proteins are deregulated in precancerous gastric lesions, suggesting their involvement in the early stages of gastric carcinogenesis, possibly due to an inflammatory process in the gastric mucosa.

Formato

11

Identificador

http://dx.doi.org/10.1155/2014/478138

Mediators Of Inflammation. New York: Hindawi Publishing Corporation, 11 p., 2014.

0962-9351

http://hdl.handle.net/11449/112844

10.1155/2014/478138

WOS:000332539600001

WOS000332539600001.pdf

Idioma(s)

eng

Publicador

Hindawi Publishing Corporation

Relação

Mediators of Inflammation

Direitos

openAccess

Tipo

info:eu-repo/semantics/article