Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
01/10/2014
01/10/2014
01/08/2013
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Resumo |
Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs. |
Formato |
668-670 |
Identificador |
http://dx.doi.org/10.1590/0074-0276108052013022 Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 108, n. 5, p. 668-670, 2013. 0074-0276 http://hdl.handle.net/11449/109882 10.1590/0074-0276108052013022 S0074-02762013000500668 S0074-0276108052013022 WOS:000322520800022 2-s2.0-84879578089 S0074-02762013000500668.pdf |
Idioma(s) |
eng |
Publicador |
Instituto Oswaldo Cruz, Ministério da Saúde |
Relação |
Memórias do Instituto Oswaldo Cruz |
Direitos |
openAccess |
Palavras-Chave | #microscopic agglutination test #prozone effect #leptospirosis |
Tipo |
info:eu-repo/semantics/article |