Influence of experimental canine ehrlichiosis on the E-ADA activity and purine levels in serum and possible functional correlations with pathogenesis


Autoria(s): Da Silva, Aleksandro S.; Munhoz, Thiago D.; Faria, Joice L.M.; Vargas-Hérnandez, Giovanni; Machado, Rosangela Z.; Luz, Nathalia C.; Moritz, Cesar E.J.; Casali, Emerson A.; Bottari, Nathieli B.; Stefani, Lenita M.; Tinucci-Costa, Mirela
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

27/05/2014

27/05/2014

25/10/2013

Resumo

The aim of this study was to evaluate adenosine deaminase activity and purines levels in serum of dogs experimentally infected by Ehrlichia canis. Banked serum samples of dogs divided into two groups with five animals each: healthy animals and animals infected by E. canis. The concentration of purines (adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), adenosine, inosine, hypoxanthine, xanthine and uric acid), and adenosine deaminase (E-ADA) activity in sera were evaluated. Samples were collected on days 12 and 30 post-infection (PI). The E-ADA activity showed a significant reduction on day 12 PI, and increased on day 30 PI in dogs infected with E. canis. On day 12, an increase in seric concentration of ATP, ADP and adenosine was verified, and different levels of hypoxanthine, xanthine and uric acid had a drastic reduction in infected compared healthy dogs (P< 0.05). However, on day 30 PI, the levels of seric ADP and AMP decreased, unlike the concentration of xanthine and uric acid that increased significantly in infected dogs (P< 0.05). Therefore, the activity of E-ADA and purine levels are altered in experimental canine ehrlichiosis, probably with the purpose of modulating the pathogenesis of the disease related to immune response, oxidative stress and coagulation disorders in acute phase. © 2013 Elsevier B.V.

Formato

602-606

Identificador

http://dx.doi.org/10.1016/j.vetmic.2013.06.033

Veterinary Microbiology, v. 166, n. 3-4, p. 602-606, 2013.

0378-1135

1873-2542

http://hdl.handle.net/11449/76882

10.1016/j.vetmic.2013.06.033

WOS:000324960200033

2-s2.0-84883290177

Idioma(s)

eng

Relação

Veterinary Microbiology

Direitos

closedAccess

Palavras-Chave #Adenosine #Adenosine deaminase #Canine #Ehrlichia canis #adenosine #adenosine deaminase #adenosine diphosphate #adenosine phosphate #adenosine triphosphate #hypoxanthine #purine #uric acid #xanthine #animal experiment #animal model #animal tissue #blood level #canine ehrlichiosis #controlled study #disease association #dog #enzyme activity #experimental infection #nonhuman #pathogenesis #protein expression #Animalia #Canis familiaris
Tipo

info:eu-repo/semantics/article